三酸 (TPhP) 的环境相关度对斑马鱼的影响发展
Benjamin Schmandt1, Mfon Diduff1, Gabrielle Smart1
1Biology Department, Bates College, Lewiston, ME 04240, USA.
Toxics
|May 24, 2024
概括
在水生环境中发现的三酸 (TPhP) 即使在环境相关的纳米分子度下,也会导致斑马鱼幼虫的发育问题. 这些影响包括缩短长度,胀和氧化应激,影响多个分子通路.
科学领域:
- 环境毒理学环境毒理学
- 发育生物学是发展生物学.
- 生态毒理学 生态毒理学
背景情况:
- 三酸 (TPhP) 是一种常见的阻燃剂和在水生生态系统中检测到的增塑剂.
- 现有的毒性研究经常使用远远超过环境相关水平 (nM) 的度 (μM).
研究的目的:
- 研究环境相关的纳米 (nM) 度TPhP对斑马鱼 (Danio rerio) 的发育影响.
- 为了确定受低水平TPhP暴露影响的分子通路.
主要方法:
- 斑马鱼幼虫长期暴露在1.5-15nm的TPhP中.
- 表型评估包括幼虫长度和心脏发育.
- 分子分析的重点是与发育途径和氧化应激相关的基因表达.
主要成果:
- 暴露于1.5-15nM的TPhP会导致幼虫的寿命缩短和心周胀.
- 观察到心脏毒性,尽管与度较高时观察到的表型不同.
- TPhP失调了tbx5a转录因子级联 (nppa,nppb,bmp4) 并充当了一个弱的亲氧化剂,增加了氧化应激.
结论:
- 在环境相关的nM度下,TPhP对斑马鱼产生不利的发育影响.
- 作用模式涉及多种途径,包括氧化应激和特定基因失调,而不是以前识别的途径,如RXR在这些低水平.
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