使用竞争性排除微生物在乳制品便基堆肥中对大肠杆菌O157:H7进行生物控制
1Department of Food, Nutrition, and Packaging Sciences, Clemson University, Clemson, SC 29634, USA.
Pathogens (Basel, Switzerland)
|May 24, 2024
概括
竞争性排斥 (CE) 微生物有效地减少了堆肥中的致病菌埃舍里希亚大肠杆菌O157:H7. 这种方法提高了堆肥的微生物安全性,最大限度地降低了土壤和作物污染风险.
科学领域:
- 农业微生物学 农业微生物学
- 环境科学 环境科学
- 食品安全 食品安全
背景情况:
- 动物肥料堆肥作为一种重要的有机肥料和土壤修补剂.
- 确保堆肥的微生物安全性对于防止病原体传播至关重要.
- 竞争性排斥 (CE) 微生物正在研究它们在堆肥中对埃舍里希亚大肠杆菌O157:H7的有效性.
研究的目的:
- 评估竞争性排斥 (CE) 微生物在乳制品便基堆肥中减少大肠杆菌O157:H7的有效性.
- 为了确定湿度和温度对CE疗效的影响.
- 在不同季节的实验室和温室条件下评估CE性能.
主要方法:
- 用大肠杆菌O157:H7和CE菌株对乳制品堆肥进行注射.
- 在实验室环境中,在不同的湿度 (20-40%) 和温度 (22°C,30°C) 下测试大肠杆菌O157:H7的减少.
- 在秋季,冬季和夏季对温室堆肥的CE有效性进行评估.
主要成果:
- 实验室条件显示,CE的大肠杆菌O157:H7的1.1-3.36日志减少,通过较高的湿度和较低的温度得到改善.
- 温室研究表明,在较寒冷的季节,在肥料中含有≥30%水分的堆肥中,CE在2天内使大肠杆菌O157:H7无活化超过99%.
- 适应热量的大肠杆菌O157:H7需要≥8天才能达到类似的降温水平.
结论:
- 竞争性排斥微生物是控制堆肥中的食物传播病原体的可行策略.
- 通过CE应用,可以显著降低堆肥对土壤和作物污染的风险.
- 优化湿度和温度条件可以提高CE对病原体控制的有效性.
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