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Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

Published on: May 23, 2019

近红外辐射测量光探针用于检测大脑内源性Cu2+

Jianping Zhu¹, Marcus E Graziotto¹, Veronica Cottam²

¹School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.

|May 24, 2024

概括

一个新的光探针CyCu1可以准确地测量生物样本中的铜 (Cu2+). 这种工具揭示了患有莱维体的痴呆症患者的大脑组织中较低的铜水平,改善了对神经系统疾病的理解.

关键词:

阿拉比多普西斯 (Arabidopsis) 是一种植物.莱维体病是莱维体病的一种疾病.基于活动的传感.铜铜铜铜的铜.氨酸是什么氨酸光探测器的光探测器神经母细胞瘤的神经母细胞瘤摄影声学成像成像技术

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科学领域:

神经科学是一个神经科学.
生物化学生物化学
医疗成像医学成像

背景情况:

铜对神经系统功能至关重要,其失调与帕金森病和阿尔茨海默病等神经系统疾病有关.
目前用于测量细胞内铜的方法缺乏灵敏度,这阻碍了对神经元疾病中铜平衡的研究.

研究的目的:

开发一种敏感的近红外 (NIR) 光探针,用于在生物系统中成像细胞内铜 (Cu2+).
使用开发的探针,研究铜失调在患有勒维体 (DLB) 痴呆症中的作用.

主要方法:

开发了一种基于反应的NIR比度光探针CyCu1,用于Cu2+检测.
应用CyCu1对细胞培养 (SH-SY5Y),植物细胞 (Arabidopsis) 和死后DLB脑组织中的Cu2+进行成像.
利用CyCu1进行了对特定脑区Cu2+分布的定量分析.

主要成果:

CyCu1表现出高稳定性和选择性,使其能够在各种生物样本中进行有效的Cu2+成像.
与对照人群相比,在DLB患者的黑体细胞质,神经元和细胞外空间中观察到Cu2+水平的显著降低.
该探测器还显示了作为双模成像应用的光声学成像剂的潜力.

结论:

CyCu1 探针是生物和病理背景下敏感的细胞内铜成像的宝贵工具.
黑色物质中的Cu2+失调与Lewy体痴呆症中观察到的神经退行有关.
这项研究促进了对铜在利维体疾病中的作用的理解,并为改善诊断成像提供了潜在的可能性.