日本脑炎病毒劫持了与ER相关的降解调节器,用于其复制
Riya Sarkar1,2,3, Simran Chhabra1, Mukesh Tanwar1
1Virology Research Group, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, 121001, India.
The Journal of general virology
|May 24, 2024
概括
日本脑炎病毒 (JEV) 的复制依赖于宿主ER相关降解 (ERAD) 蛋白. 削减这些ERAD因子,特别是SEL1L,显著抑制了JEVRNA复制和病毒标称.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 弗拉维病毒,包括日本脑炎病毒 (JEV),在内质网膜 (ER) 中复制.
- 病毒和宿主蛋白质对于弗拉维病毒复制复合物的形成和功能至关重要.
- 与ER相关的降解 (ERAD) 途径在ER内的蛋白质稳态中发挥作用.
研究的目的:
- 研究ER相关降解 (ERAD) 途径蛋白在日本脑炎病毒 (JEV) 复制中的作用.
- 确定JEV感染期间ERAD组件的局部化和功能意义.
主要方法:
- 高分辨率免疫光成像JEV感染的HeLa细胞.
- 分析病毒蛋白 (NS1,NS5) 和ERAD蛋白质的局部化.
- 对ERAD效应因子的转录和蛋白质水平分析.
- 通过siRNA介导的ERAD蛋白质 (OS9,SEL1L,HERPUD1,HRD1) 的耗尽.
- 病毒RNA复制和标位量化.
- 蛋白质翻译停止实验.
主要成果:
- 与ERAD蛋白质SEL1L,OS9,HERPUD1,HRD1和DERLIN1.1结合在一起的JEV复制复合体 (NS1) 进行同位体化.
- SEL1L也与NS5阳性结构重叠.
- 虽然ERAD效应基因被转录上调,但蛋白质水平保持稳定.
- 缺少OS9,SEL1L,HERPUD1和HRD1显著降低了病毒RNA复制和标位.
- SEL1L的减少导致了JEV复制的最实质性的抑制.
- 在JEV感染期间,SEL1L和OS9蛋白水平稳定.
结论:
- ERAD通路蛋白质SEL1L,OS9,HERPUD1和HRD1是JEV复制的关键宿主因素.
- 这些ERAD蛋白对于有效的病毒RNA合成和生产至关重要.
- JEV感染会影响特定ERAD组件的稳定性和功能.
关键词:
德林 (DERLIN) 是一个名为DERLIN的小镇.欧洲议会 (ERAD) 委员会.赫尔普德 (HERPUD) 是一个人力资源发展计划1 HRD1在OS9中,它是OS9.这就是SEL1L的意义.弗拉维病毒 (flavivirus) 是一种病毒.反向转换的区块链无处不在的蛋白质酶体更多相关视频
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