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Updated: Jun 25, 2025

Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide
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甲基组的标记:基于分子量选择H前体的简化协议和指导

Alexandra Locke1, Kylee Guarino1, Gordon S Rule2

  • 1Department of Biological Sciences, Carnegie Mellon University, 4400 5th Ave, Pittsburgh, PA, 15213, USA.

Journal of biomolecular NMR
|May 24, 2024
PubMed
概括

本研究介绍了一项用于核磁共振 (NMR) 研究的蛋白质甲基标记的快速,一天的协议. 大型蛋白质 (高达150kDa) 的高质量的NMR光谱可以在没有昂贵的化溶剂或葡萄糖的情况下实现.

关键词:
细菌的表达方式生物物理学的生物物理.生物技术是生物技术.这就是NMR的NMR.蛋白质结构 蛋白质结构

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¹H NMR of Labile Protons: Deuterium (²H) Substitution

This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
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Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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