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Updated: Jun 25, 2025

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

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基于正交选择性记者的人类多能干细胞无痕基因组编辑协议

Yun Zhao1, Zihang Pan1, Zixuan Hong1

  • 1Department of Physiology and Pathophysiology, School of Basic Medical Sciences, State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing 100191, China.

STAR protocols
|May 24, 2024
PubMed
概括

这项研究引入了一种用于精确编辑人类多能干细胞 (hPSCs) 的新协议. 该方法使用可视化记者来有效地创建用于研究和治疗的转基因hPSC线.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?细胞分化的过程流动细胞计量 (Flow Cytometry) 是一种流动细胞计.分子生物学分子生物学干细胞是干细胞.

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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