相关实验视频

Updated: May 2, 2026

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Published on: December 9, 2017

关于在体外识别OXCT1介导的LACTB糖化位点的协议

Wenhao Ma¹, Yuchen Sun¹, Huafeng Zhang²

¹Anhui Key Laboratory of Hepatopancreatobiliary Surgery, Department of General Surgery, Anhui Provincial Hospital, The First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, Anhui 230027, China; The Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, Anhui 230027, China; Anhui Province Key Laboratory of Biomedical Aging Research, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, Anhui 230027, China.

|May 24, 2024

概括

这项研究引入了一项新协议,用于测量关键蛋白质修饰的关键蛋白质修饰. 该方法使用西方抹杀和质谱法来检测LACTB蛋白上的化水平和位点,由OXCT1.1介导.

科学领域:

生物化学生物化学
蛋白质组学是指蛋白质组学.
分子生物学分子生物学

背景情况:

蛋白质化是一种关键的翻译后修改.
氧化性1-3-基酸盐载体蛋白1 (OXCT1) 作为糖转移酶起作用.
血清蛋白β-乳糖酶样蛋白 (LACTB) 是OXCT1介导的化的目标.

研究的目的:

为量化OXCT1介导的LACTB糖化提出一个详细的协议.
在LACTB上确定特定的化位点.
建立一种适用于其他蛋白质的方法.

主要方法:

西部涂抹 (WB) 用于检测化水平.
质谱仪 (MS) 用于识别化部位.
在体外测试以研究OXCT1-LACTB相互作用.

主要成果:

成功检测了OXCT1介导的LACTB糖化水平.
确定K284作为LACTB上的化部位.
验证该协议对其他蛋白质的适用性.

结论:

开发的协议允许精确测量蛋白质化.
这种方法有助于理解OXCT1在LACTB修改中的作用.
该协议是蛋白质组研究的宝贵工具.

关键词:

细胞生物学细胞生物学基于细胞的测定.蛋白质生物化学蛋白质生物化学

更多相关视频

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

Published on: April 4, 2018

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

相关概念视频

Formation of Lipopolysaccharides

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...