坎比洛巴克特 (Campylobacter jejuni) 和素水解剂的添加:对家禽体外细胞微生物群和代谢组的影响
E G Olson1, D K Dittoe2, C C Chatman3
1Department of Animal and Dairy Sciences, Meat Science and Animal Biologics Discovery Program, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
PloS one
|May 24, 2024
概括
素水解剂在存在Campylobacter jejuni时改善了家禽肠道微生物群和维生素B的产生. 微生物群体对特定的素来源表现出偏好,影响了肠道健康和竞争动态.
科学领域:
- 微生物学 微生物学
- 动物营养 动物营养
- 胃肠道健康 胃肠道健康
背景情况:
- 在家禽中植根Campylobacter jejuni菌CECA可能会对肠道健康产生负面影响.
- 了解微生物对食蛋白质来源的反应对于家禽健康管理至关重要.
- 素水解剂代表了一个潜在的营养策略来调节肠道微生物群.
研究的目的:
- 调查不同素水解剂对家禽菌微生物群组成和功能在Campylobacter jejuni.存在时的影响.
- 为了确定微生物分子偏好完整的素,酶解酸和酸解酸.
- 评估素补充剂对微生物多样性,丰富性和代谢物产生的影响,特别是B族维生素.
主要方法:
- 一个体外模型系统,使用用C. jejuni.接种的家禽肉含量.
- 用完整的素 (IN),酶化剂 (EH) 和酸化剂 (AH) 化样品48小时.
- 16S rRNA基因测序用于微生物社区分析和超高性能液态染色体质谱法 (UHPLC-MS) 用于代谢分析.
主要成果:
- 在酶化 (EH) 组中,C. jejuni的丰度最高.
- 素补充剂通常会改善微生物多样性和丰富性.
- 观察到维生素B相关代谢物的显著增加,在EH和AH组之间,维生素B6和B9的不同模式. 体细菌和Phascolarctobacterium分别与AH和EH组相关.
结论:
- 在家禽中,微生物群落的结构和功能受到素水解剂类型和C. jejuni存在的影响.
- 素水解剂可以增强维生素B的产生,调节微生物的竞争动态.
- 使用特定的素水解剂的营养干预措施显示出改善家禽肠道微生物群和健康结果的潜力.
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