Ugd是一种囊型多糖合成蛋白质,它调节了Vibrio alginolyticus中的细菌运动性
Xixi Li1, Xingyi Fei1, Qingwei Chen1
1Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization, Hohai University, Nanjing 210098, China; Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, China.
Microbiological research
|May 24, 2024
概括
维布里奥·阿尔金诺利蒂克斯 (Vibrio alginolyticus) 群体运动与囊多糖 (CPS) 合成有关. ugd基因删除突变显示了CPS的减少,运动能力受损和基因表达的改变,这表明ugd是V. alginolyticus生理学的关键.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 病原体研究 病原体研究
背景情况:
- 菌菌是一种重要的机会性病原体,影响海洋生物和人类.
- 了解V. alginolyticus毒性和运动性的遗传基础对于开发控制策略至关重要.
研究的目的:
- 为了确定调节V. alginolyticus. 群体运动性的基因.
- 研究囊多糖 (CPS) 合成基因ugd在V. alginolyticus生理学和毒性的作用.
主要方法:
- 构建和选一个V. alginolyticus转子突变库.
- 基因删除,补充和表型测定 (运动性,生物膜,细胞毒性).
- 转录组分析 (RNA-Seq),KEGG通路分析和RT-qPCR.
主要成果:
- 鉴定了影响群体运动的鞭毛和CPS合成基因; ugd被确认为关键.
- 格突变体表现出降低的CPS,游泳能力受损,生物膜形成减少,细胞毒性增加.
- 转录组分析显示,双组分系统 (TCS),化学反应和鞭毛组装基因的显著下调,以及人类疾病和代谢相关基因的上调.
结论:
- 该ugd基因在V. alginolyticus群动性,CPS产生,生物膜形成和毒性方面发挥着至关重要的作用.
- Ugd 影响了涉及TCS,化学反应,鞭毛组合和新陈代谢的基因表达.
- 乌格德代表了针对V. alginolyticus感染的治疗干预的潜在目标.
相关概念视频
Cytoskeletal Proteins in Bacteria
3.4K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.4K
Intracellular Movement of Viruses and Bacteria
2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K
Actin Polymerization and Cell Motility
5.2K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.2K
Bacterial Signaling
32.0K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
32.0K
Cell Motility through Blebbing
1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
1.9K
GPCRs Regulate Adenylyl Cylase Activity
5.5K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.5K


