控制III/VI型分泌系统的长链不和脂肪酸传感器对于Edwardsiella piscicida感染至关重要
Feng Xia1, Yihan Liu1, Lifan Wei1
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Microbiological research
|May 24, 2024
概括
不和脂肪酸 (UFA) 通过与EsrC.交互,在海洋病原体Edwardsiella piscicida中关闭了必不可少的毒性因素. 这种相互作用对E. piscicida感染和宿主中殖民至关重要.
科学领域:
- 微生物学 微生物学
- 病原体生物学 病原体生物学
- 水产养殖健康 水产养殖健康
背景情况:
- Edwardsiella piscicida是一种重要的海洋病原体,影响着全球水产养殖.
- 病原发生在很大程度上依赖于III型 (T3SS) 和VI型 (T6SS) 分泌系统,由EsrB和EsrC调节.
研究的目的:
- 研究脂肪酸对E. piscicida毒性因子表达的影响.
- 阐明脂肪酸调节T3SS和T6SS的机制.
主要方法:
- 研究脂肪酸与调节蛋白EsrC.的相互作用.
- 利用突变菌株 (esrCR38G) 来评估功能影响.
- 检查了宿主细胞 (HeLa) 和模型生物 (斑马鱼) 中的细菌殖民.
主要成果:
- 不和脂肪酸 (UFA),但不是和脂肪酸 (SFAs),直接与EsrC结合,抑制T3/T6SS.
- 主体UFA通过FadL被运送到E. piscicida中,在感染期间调节T3/T6SS表达.
- 一个无法结合UFA的突变体显示出生长和殖民能力受损.
结论:
- 在E. piscicida中,UFA-EsrC相互作用是降低T3/T6SS调节的关键机制.
- 这种调节途径对于有效的E. piscicida感染和殖民是必不可少的.
关键词:
爱德华兹鱼 (Edwardsiella piscicidacida) 是一种鱼类生长的植物.这是一个很棒的经验.鱼类病原体的病原体这就是T3SS.在T6SSSS中使用.不和脂肪酸UFA 不和脂肪酸UFA更多相关视频
08:36Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth
Published on: May 31, 2024
446
10:29Applying Fluorescence Resonance Energy Transfer FRET to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
Published on: July 6, 2016
10.7K
相关概念视频
Bacterial Signaling
32.0K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
32.0K
Adherens Junctions
4.7K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
4.7K
