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全转录组RNA测序为泥的卵巢发育的分子动力学提供了新的见解,在交配后的泥,Scylla paramamosain的卵巢发育

Yang Yu¹, Mengqian Zhang², Dahe Wang¹

¹Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; International Joint Research Center for the Development and Utilization of Important Mariculture Varieties Surrounding the South China Sea Region, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China; Higher Institute Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus 21030, Terengganu, Malaysia.

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概括

交配通过促进快速成熟,显著加快了泥的卵巢发育. 这项研究确定了关键的基因和分子通路,如氨酸 - 血管新生素系统,参与了这一关键的生殖过程.

科学领域:

海洋生物学海洋生物学
甲动物的繁殖甲动物的繁殖
分子内分泌学分子内分泌学

背景情况:

卵巢发育是一个复杂的过程,受到各种因素的影响.
了解像泥 (Scylla paramamosain) 等甲类的卵巢成熟的分子机制对于水产养殖和生殖生物学至关重要.

研究的目的:

研究交配对泥卵巢发育的影响.
为了确定差异表达的基因,非编码RNA和参与交配诱导的卵巢成熟的调节途径.

主要方法:

来自交配和不交配的雌性泥的卵巢组织的组织学分析.
全转录组测序 (mRNA,lncRNA,circRNA,miRNA) 用于识别差异表达的分子.
竞争的内源性RNA (ceRNA) 网络的构建.
丰富信号通路的生物信息分析和基因本体学 (GO) 术语.

主要成果:

交配显著加快卵巢成熟,交配的雌性比未交配的雌性表现出更大的卵子和更快的进展到第三阶段.
总共有518个mRNAs,1502个lncRNAs,18个circRNAs和151个miRNAs在交配阶段之间被差异地表达.
确定了与卵巢发育相关的六个关键基因,包括那些参与激素受体和细胞循环调节的基因.
丰富的途径包括氨酸 - ангиотензин系统,TGF-β信号传递,细胞粘附分子,MAPK信号传递和ECM受体相互作用.
ceRNA网络突出了与发展过程,繁殖和生长相关的GO术语.

结论:

交配对于泥的卵巢快速发育至关重要.
特定的基因和分子通路,特别是氨酸-血管酶系统和TGF-β信号传递,在调节伴侣诱导的卵巢成熟方面发挥着关键作用.
这些发现为加强雌性泥成熟的策略提供了基础,并加深了对类生殖生物学的理解.

关键词:

人工交配是一种人工交配.在CeRNA网络中,卵巢发育卵巢发育斯基拉帕拉马莫萨因 (Scylla paramamosain) 是一个古老的植物.整个转录基因组的测序.

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