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哈普顿合成,单克隆抗体的产生和免疫试验的发展,以分析螺旋的残留物

Yaqi Jin¹, Zihui Jin¹, Di Sun¹

¹Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400712, China; National Citrus Engineering Research Center, Chongqing 400712, PR China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture, Citrus Research Institute, Southwest University, Chongqing 400712, China.

|May 24, 2024

概括

开发了一种新的免疫试验,用于检测一种强大的杀虫剂和杀虫剂spiropidion. 该方法精确监测食品和农业样本中的螺旋残留物,确保安全性和合规性.

科学领域:

农业化学农业化学
免疫技术是一种免疫技术.
虫害管理虫害管理虫害管理

背景情况:

斯皮罗皮迪翁对各种吸虫害具有显著的杀虫和杀虫性质.
对螺旋残留物的有效监测对于食品安全和农业实践至关重要.

研究的目的:

开发一种灵敏可靠的免疫检测方法来检测螺旋.
在现实世界农业和环境样本中验证试验的性能.

主要方法:

基于spiropidion的 haptens 的合成.
通过混合体技术产生特定的单克隆抗体 (mAb 8B5).
建立和优化异质间接竞争性酶相关免疫吸收试验 (ic-ELISA).

主要成果:

开发的ic-ELISA显示出高灵敏度,IC50为7.36 ng/mL,工作范围为1.75-34.92 ng/mL.
该测试在环境 (长江) 和食品 (类) 样本中显示出很好的准确性,平均恢复率在76.05%至124.78%之间.
来自ic-ELISA的结果与使用样品的超高性能液体染色学-并联质谱法 (UPLC-MS/MS) 获得的结果相关性很好.

结论:

已建立的ic-ELISA是监测螺旋残留物的可行工具.
这种方法可用于确保食品和农产品的安全.
该试验提供了在各种样本矩阵中进行残留分析的实用方法.

关键词:

发生的事情发生了.伊克-埃利萨 (Ic-ELISA) 是一个有趣的小组.单克隆抗体是一种单克隆抗体.农药残留物农药残留物斯皮罗皮迪翁 (Spiropidion) 是一种的生物.

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