来自Lactiplantibacillus plantarum的新型内糖酶的表征
Sahara Sarang1, Luise Ernst2, Daniel Wefers2
1Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Pune 412115, India.
Food chemistry
|May 24, 2024
概括
研究人员特征了一种来自Lactiplantibacillus plantarum DKL3的新型果糖糖酶,产生一种独特的胰岛素类型果糖素. 这种酶,LpInu,由于其独特的特性,显示出食品和生物医学应用的潜力.
科学领域:
- 酶学 是一种酶学.
- 微生物学 微生物学
- 碳水化合物化学 碳水化合物化学
背景情况:
- 果糖糖酶从糖糖中合成果糖.
- 一种来自Lactiplantibacillus plantarum DKL3的新型果糖糖酶被确定.
- 这种酶与已知的果糖糖酶具有较低的序列相似性.
研究的目的:
- 从L. plantarum DKL3.3中重新组合表达和表征独特的果糖糖酶.
- 为了确定酶的最佳条件和基质特异性.
- 评估酶及其产品的潜在应用.
主要方法:
- 果糖糖酶的重组表达和净化.
- 酶的表征包括pH值,最佳温度和金属离子效应.
- 使用NMR光谱和甲基化分析对产生的果进行结构分析.
- 通过高性能尺寸排除色谱与折射率检测 (HPSEC-RI) 来确定分子重量.
主要成果:
- 这种名为LpInu的酶在pH值为4.0和35°C时运行得最好.
- 酶活性由Ca2+和Mn2+显著增强.
- LpInu产生一种具有β-2,1链接和一些β-2,1,6分支点的胰岛素类型的果糖.
- 由此产生的胰岛素与其他细菌胰岛素相比,其分子量较低.
结论:
- 乳植物菌植物 DKL3 含有一个独特的 inulosucrase (LpInu).
- 由LpInu产生的特征性胰岛素具有独特的结构特征和较低的分子量.
- LpInu及其胰岛素产品是食品和生物医学应用的有希望的候选者.
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