乳糖酶的连续和自主流程分离使用功能化的水性双相系统与计算机视觉控制
Vikas Sharma1, Jeong-Un Joo1, Amirreza Mottafegh1
1Center for Intelligent Microprocess of Pharmaceutical Synthesis, Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
Bioresource technology
|May 24, 2024
概括
这项研究介绍了一种新的囊功能化水性双相系统 (ATPS),用于高效的乳糖酶提取. 微流体技术提高了生物制药净化中的分区系数和提取效率.
科学领域:
- 生物化学工程 生物化学工程
- 分离科学 分离科学
- 生物技术是生物技术.
背景情况:
- 生物分子的下游加工,特别是治疗性蛋白质和酶,是昂贵而复杂的.
- 目前的方法往往涉及复杂的单元操作,限制了效率.
研究的目的:
- 开发一种新型的氨酸 (cys) 功能化水性双相系统 (ATPS) 用于选择性乳糖酶提取.
- 将该系统集成到连续流动的微流体装置中,用于自动生物分离.
主要方法:
- 一种基于聚乙烯甘醇 (PEG) 和酸 (K3PO4) 的ATPS被cysteine功能化.
- 一个3D-baffle微混合器和具有计算机视觉控制的相分离器被设计用于自动化,连续流动的操作.
- 拉卡斯提取效率和分割系数与传统批量工艺进行了比较.
主要成果:
- 微流体辅助ATPS (PEG-K3PO4/cys) 获得了16.3的分区系数 (Kflow) 和88%的提取效率 (EEflow).
- 连续流程过程表明,即使在低度下,从复杂的混合物中有效分离乳糖酶.
- 循环二重化证实了在过程中乳糖酶的结构稳定性.
结论:
- 使用功能化的ATPS的连续流微流体生物分离为下游加工提供了高效和自动化的解决方案.
- 这种方法对于无净化目标生物制药非常有价值.
- 开发的系统可以实现集成和自主生物分离过程.
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