增强来自Schizochytrium sp.的多可萨赫萨酸的产生. 通过使用废弃的Pichia pastoris作为源,基于两阶段的养控制
Luqiang Jia1, Tianyi Li1, Ruoyu Wang1
1School of Food Science and Technology, Yangzhou University, 225127 Yangzhou, China.
Bioresource technology
|May 24, 2024
概括
使用废弃物Pichia pastoris作为源,可以促进Schizochytrium sp.中的多可沙赫酸 (DHA) 生产. "DO足量-限"策略显著增加了DHA产量,增加了1.58倍.
科学领域:
- 生物技术是生物技术.
- 微生物发酵 微生物发酵
- 生物化学工程 生物化学工程
背景情况:
- 在工业应用中,降低多可胺酸 (DHA) 生产成本至关重要.
- 精神分裂 (Schizochytrium sp.) 是一种疾病. 是DHA生物合成的关键微生物.
- 使用像Pichia pastoris这样的废弃产品可以提高可持续性并降低生产成本.
研究的目的:
- 调查废弃物Pichia pastoris的使用,作为一种替代性源,用于高密度种植Schizochytrium sp.
- 开发和比较控制策略,以优化在高密度条件下的DHA生产.
- 通过高效的发酵控制来最大限度地提高DHA度和产量.
主要方法:
- 在高密度种植Schizochytrium sp.的种植. 使用Pichia pastoris作为一个源.
- 实施和评估两个控制策略:"DO 充足度-限制"和"DO 限制-充足度".
- 测量DHA生物合成途径中的葡萄糖维持系数和酶活性.
主要成果:
- 与对照组 (17.0 mg/g/h) 相比",DO充足度-限制"策略导致较低的葡萄糖维持系数 (12.3 mg/g/h).
- 这一策略还导致了参与DHA生物合成的酶的活动增加.
- 达到了12.8±1.2g/L的最大DHA度,比对照组增加1.58倍.
结论:
- 废物Pichia pastoris是一种有效的替代源,可以节省成本生产DHA.
- 在优化DHA发酵方面",DO足量-限制"策略优越.
- 替代源的双阶段养控制是工业DHA发酵的可行和高效方法.
相关概念视频
01:18Bioreactor Controls-II
82
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
82
01:22Bioreactor Controls-III
70
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
70
01:30Designing Growth Media for Bioreactors
84
Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
84
01:27Upstream Processing
102
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
102
01:25Production of Organic Acids
111
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
111