光度测试和细菌学测试的有效性,用于诊断乳牛在干燥时的乳腺内感染
J Denis-Robichaud1, N Barbeau-Grégoire2, M-L Gauthier3
1Independent researcher, Amqui, Québec, Canada G5J 2N5.
Journal of dairy science
|May 24, 2024
概括
这项研究发现,实验室培养,Petrifilm和Tri-Plate测试对于在干燥时在奶牛中识别乳腺内感染 (IMI) 是有效的,与光度测试不同. 细菌学测试为乳牛群中IMI提供了可靠的诊断.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 乳制品科学 乳制品科学
- 诊断微生物学 诊断微生物学
背景情况:
- 在干燥的乳牛中,精确诊断乳腺内感染 (IMI) 对于有效的群体管理和牛奶质量至关重要.
- 使用各种诊断方法,包括实验室培养,农场培养系统 (Petrifilm,Tri-Plate) 和光度测量,但它们的比较有效性尚未完全确定.
研究的目的:
- 为了估计实验室培养,Petrifilm,Tri-Plate和光度的诊断有效性 (灵敏度和特异性),用于在干燥时检测奶牛的IMI.
- 评估这些测试的预测值和错误分类成本,在不同的流行情景和虚假阴性与虚假阳性比率下.
主要方法:
- 一项涉及333头荷尔斯坦乳牛在干燥前的横截面研究.
- 牛奶样本被分为光度测量,petrifilm,三板和实验室培养.
- 用贝叶斯隐性类分析来估计测试性能,考虑到所有测试都是不完美的.
主要成果:
- 光度测试表明,在干燥时检测IMI的有效性很差.
- 实验室培养 (Se: 0.93,Sp: 0.69),Petrifilm (Se: 0.91,Sp: 0.71) 和Tri-Plate (Se: 0.65,Sp: 0.85) 显示出足够的有效性.
- 细菌学测试具有相似的积极预测值,但Tri-Plate的负预测值低于实验室培养和Petrifilm,IMI患病率更高.
结论:
- 细菌学测试 (实验室培养,Petrifilm,Tri-Plate) 是有效的诊断IMI在奶牛在干.
- 光度不是一个可靠的方法来检测IMI在干燥.
- 石膏膜和三板提供与实验室培养相比较的性能,适用性取决于患病率和虚假阴性与虚假阳性的相对重要性.
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