化学物质对神经网络活动的影响:微电极阵列试验中急性暴露与网络形成暴露的比较
Melissa M Martin1, Amy F Carpenter2, Timothy J Shafer1
1Computational Toxicology & Bioinformatics Branch, Biomolecular and Computational Toxicology Division, CCTE/ORD, US. Environmental Protection Agency, Research Triangle Park, NC 27711, USA.
Toxicology
|May 24, 2024
概括
使用微电极阵列 (MEA) 的新方法方法在评估化学神经毒性方面显示出高度一致性. 结合急性网络功能试验 (AcN) 和网络形成试验 (NFA),可以为危险识别提供全面的评估.
科学领域:
- 神经科学是一个神经科学.
- 毒理学 毒理学 毒理学
- 测试开发 测试开发
背景情况:
- 新方法方法 (NAM) 对于评估缺乏数据的化学品的神经毒性和发育神经毒性至关重要.
- 微电极阵列 (MEA) 提供了一种用于危险评估的神经活动测量技术.
- 已经开发了两种基于MEA的试验,即急性网络功能试验 (AcN) 和网络形成试验 (NFA).
研究的目的:
- 评估MEA AcN和NFA之间的一致性,以评估化学神经毒性.
- 为了确定影响AcN的化学物质是否也会扰乱NFA.
- 为化学评估中使用这两种测试提供理由.
主要方法:
- 使用MEA AcN和NFA选243种化学物质.
- 评估两个试验之间的定量和定性一致性.
- 分析选择性活动,功效和生物活性指纹.
主要成果:
- 在排除细胞毒性活动后,在AcN和NFA之间观察到70.3%的一致性.
- 国家药物管理局检测到更多与细胞毒性相关的活性化学物质.
- AcN和NFA提供了独特的生物活性指纹,AcN有助于识别作用模式,NFA提供了发育暴露的信息.
结论:
- 无论是MEA AcN还是NFA都是化学神经毒性评估的宝贵工具.
- 结合AcN和NFA提供了一个比单独测试更全面的评估.
- 测试的选择取决于具体的应用,如查,危险识别或机制研究.
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