1型糖尿病人体肠状体研究显示,肠道上皮层部分发生了重大变化
Vishwesh Bharadiya1, Yan Rong1, Zixin Zhang1
1Divisions of Gastroenterology and Hepatology, Department of Medicine, the Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
Scientific reports
|May 24, 2024
概括
儿童1型糖尿病 (T1D) 患者表现出明显的十二指甲上皮变化. 这些在人体肠状体中的发现突出显示了肠道功能异常,并为研究T1D胃肠道并发症提供了一个新的模型.
科学领域:
- 胃肠病学 胃肠病学
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
背景情况:
- 在1型糖尿病 (T1D) 中胃肠道 (GI) 并发症的了解很少.
- 人类肠体是肠表皮研究的有价值的模型.
- 从T1D患者中确定体是具有挑战性的.
研究的目的:
- 为了确定和描述儿科T1D患者的十二指肠肠.
- 为了将T1D体与健康人群 (HS) 的体进行比较.
- 调查T1D中胃肠道并发症的病理生理学.
主要方法:
- 从儿科T1D患者中成功建立了十二指肠肠.
- 与HS.的年龄匹配的体动物进行比较.
- 大量RNA测序 (RNA-seq) 和功能性离子运输分析.
主要成果:
- 与细胞分化,增殖和刷边缘膜相关的基因的显著差异.
- 在T1D中增加了肠内分泌细胞和Ki-67表达.
- 降低了NHE3表达,降低了上皮屏障完整性,并在T1D中增加了液体分泌.
结论:
- 儿科T1D十二指肠体表现出异常的肠上皮的特征.
- 这些体有别于HS体.
- 儿科内类药物作为T1D胃肠道并发症研究和药物发现的可行的活体模型.
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