相关实验视频

Updated: Jun 25, 2025

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

一个双向非编码RNA促进体调解长距离基因表达调节

Carlos Alberto Peralta-Alvarez^1,2, Hober Nelson Núñez-Martínez¹, Ángel Josué Cerecedo-Castillo¹

¹Instituto de Fisiología Celular, Departaménto de Genética Molecular, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.

|May 25, 2024

概括

双向基因促进体调节基因表达超出了局部效应. 这项研究表明,一种特定的双向促进体通过促进体-促进体相互作用影响远程基因BBX表达.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

人类基因促进体表现出超出局部转录的复杂调节作用.
双向促进体在哺乳动物基因组中很常见,它们调节基因对.
双向促进器的全部功能范围在很大程度上仍未得到研究.

研究的目的:

为了研究长非编码RNA基因促进体的长距离基因表达调节功能.
探索促进剂-促进剂相互作用在基因调节中的作用.

主要方法:

采用了染色体形状捕获 (3C) 技术.
分析的重点是特定的双向促进剂及其与远端基因BBX促进剂的相互作用.
在基因上下文操纵后,评估了基因表达水平和促进剂-增强剂相互作用.

主要成果:

发现一种双向促进剂通过促进剂-促进剂相互作用以特定的目标方式调节远程基因表达.
双向促进体和BBX促进体之间的相互作用被验证为BBX转录的调制剂.
移除双向促进剂改变了BBX促进剂-增强剂相互作用,导致基因表达的增加.
这种远程调节独立于相关的非编码基因的表达水平.

结论:

双向促进体可以对基因表达施加长距离的调控控制.
由双向促进体介导的促进体-促进体相互作用是远端基因调节的关键机制.
双向促进体的功能后果超出了它们的直接基因组周围.

关键词:

双向推广者是一个双向推广者.这是一个 cis-regulatory 元素.基因结构基因结构长非编码RNA是什么意思转录法规转录法规转录法规

更多相关视频

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

The Eukaryotic Promoter Region

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...