定义一个包含LCORL-NCAPG位置的哈普洛型,与肉牛中增长的瘦生长相关
Leif E Majeres1, Anna C Dilger2, Daniel W Shike2
1UTIA Genomics Center for the Advancement of Agriculture, Institute of Agriculture, University of Tennessee, Knoxville, TN 37996, USA.
Genes
|May 25, 2024
概括
在LCORL-NCAPG位点的遗传变异显著影响肉牛的生长. 全基因组测序确定了候选变异,包括LCORL中的框架转移突变,与增长特征密切相关.
科学领域:
- 动物遗传学动物遗传学
- 畜牧科学 畜牧科学 畜牧科学
- 分子生物学分子生物学
背景情况:
- 众所周知,LCORL-NCAPG基位会影响肉牛的生长特征.
- 确定特定的致病性遗传变异仍然是一个挑战.
研究的目的:
- 确定与肉牛增长相关的LCORL-NCAPG位点内的候选致病变异.
- 改进与生长相关的单元型的关键区域.
主要方法:
- 全基因组测序的夏洛莱-sired小牛 (QQ和qq基因型).
- 在精细的单元型区域内的变异的识别和表征.
- 跨多个牛种群的LCORL编码序列变异的基因定型.
主要成果:
- 一个含有218个变异的814 kb区域被确定为Q单元型区域.
- 候选变体包括NCAPG中的内基插入,已知的NCAPG突变,两个LCORL编码突变 (rs109696064,rs384548488) 和监管变体.
- 这种LCORL位变体rs384548488可能会导致功能丧失.
- 在LCORL变异与出生体重,活体重,尸体体重,部高度和平均每日增长之间发现了显著的关联 (p < 0.05).
结论:
- 这项研究证实了LCORL-NCAPG位点与肉牛生长之间的关联.
- 特定的DNA变异,包括LCORL中的框架转移突变,被定义并与生长表型联系在一起.
- 需要进一步的研究来确定确切的致病突变.
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