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在Eriocheir sinensis中引起的氧化应激反应:抗氧化防御和免疫基因表达动态

Qinghong He¹, Wenrong Feng², Xue Chen²

¹College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China.

Antioxidants (Basel, Switzerland)

|May 25, 2024

概括

氧化 (H2O2) 暴露在 * Eriocheir sinensis * 中会影响抗氧化酶和免疫基因表达,揭示细胞应激反应,这对水产养殖中的疾病管理至关重要.

科学领域:

水生物质毒理学研究
甲动物生理学甲动物生理学
氧化应激研究研究氧化应激研究

背景情况:

*Eriocheir sinensis* 水产养殖容易受到与氧化压力相关的疾病的影响.
了解对氧化压力的反应对于疾病预防和治疗至关重要.

研究的目的:

为了研究Eriocheir sinensis对过氧化 (H2O2) 诱导的氧化应激的生理和分子反应.
阐明在的疾病易感性背后的生物过程.

主要方法:

(*Eriocheir sinensis*) 暴露于不同度和时间的H2O2.2.
测量了抗氧化酶活动 (SOD,CAT,T-AOC,GSH,GSH-Px) 和氧化损伤标志物 (MDA,H2O2,8-OHdG).
用于防御和免疫的基因表达水平 (SOD,CAT,GSH-Px,proPO,PX,HSP70,iNOS) 通过mRNA分析进行分析.

主要成果:

抗氧化酶活性最初增加,然后下降,SOD,GSH-Px和T-AOC在96小时下降到控制水平以下.
氧化损伤标志物 (MDA,H2O2,8-OHdG) 随着H2O2暴露时间的增加而增加.
抗氧化酶 (SOD,CAT,GSH-Px) 的基因表达在72小时达到峰值,而免疫基因 (proPO,PX,HSP70,iNOS) 显示出不同的反应,在高H2O2水平下抑制了proPO.

结论:

H2O2对Eriocheir sinensis的氧化状态和免疫系统产生重大影响.
细胞应激反应机制对于的健康和抗病能力至关重要.
研究结果为制定减轻氧化应激和改善养殖水产养殖疾病管理的战略提供了见解.

关键词:

埃里奥切尔·辛内斯尼斯 (Eriocheir sinensis) 是一座山脉中的一个.在H2O2中,H2O2是H2O的.抗氧化作用抗氧化作用基因表达的基因表达方式氧化应激是一种氧化应激.

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