二甲基硫氧化物 (DMSO) 作为干扰与硫代谢相关研究的潜在来源 - - 一项初步研究
Marta Kaczor-Kamińska1, Kinga Kaszuba1, Anna Bilska-Wilkosz1
1Chair in Medical Biochemistry, Faculty of Medicine, Jagiellonian University Medical College, Kopernika 7 St., 31-034 Krakow, Poland.
Antioxidants (Basel, Switzerland)
|May 25, 2024
概括
甲基二硫氧化物 (DMSO) 显著影响细胞培养中的硫代谢,即使在接受的水平. 这些复杂的效应干扰研究,需要在生物学研究中仔细考虑.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 代谢学 代谢学 代谢学
背景情况:
- 硫酸二甲基 (DMSO) 是生物研究中常见的有机硫溶剂,广泛用于细胞培养和体内研究.
- 它对硫代谢参数的潜在干扰经常被忽视.
- 粘多糖症研究通常涉及改变硫代谢的细胞系.
研究的目的:
- 研究DMSO对细胞培养中的硫代谢参数的影响.
- 为了确定DMSO干扰是否可以通过将其封装在聚合体中来减轻.
主要方法:
- 利用了三个细胞系,包括Caco-2和两个粘多糖的模型.
- 在DMSO添加到培养基后评估硫代谢参数.
- 进行了用DMSO封装在聚合体 (PEG-PLGA) 中的实验,以评估细胞膜效应的缓解.
主要成果:
- 即使在普遍接受的度下,添加DMSO也会改变硫代谢的关键参数.
- 观察到复杂的协同效应,而不仅仅是简单的添加干扰.
- 将DMSO部分加载到聚合体中显示出减轻某些破坏性影响的潜力.
结论:
- 在专注于硫代谢的研究中,DMSO是干扰的重要来源.
- DMSO的影响很复杂,不能总是通过减去对照值来纠正.
- 研究人员必须考虑DMSO对硫代谢的影响,以确保实验有效性.
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