优化高密度发酵条件的Saccharomycopsis fibuligera Y1402通过响应表面分析
Hongyang Yuan1, Qi Sun1, Lanshuang Wang1
1Key Laboratory of Geriatric Nutrition and Health (Beijing Technology and Business University), Ministry of Education, Beijing 100048, China.
Foods (Basel, Switzerland)
|May 25, 2024
概括
使用统计方法优化了Saccharomycopsis fibuligera的高密度发酵. 这导致殖民地数量增加了31倍,使工业应用成为可能.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 发酵科学 发酵科学
背景情况:
- 糖菌素 (Saccharomycopsis fibuligera) 对于发酵食品至关重要,产生酶和风味化合物.
- 有限的高密度发酵阻碍了其工业用途.
研究的目的:
- 为S. fibuligera Y1402.2的高密度培养优化介质组成和发酵条件.
- 提高S. fibuligera的工业应用性.
主要方法:
- 采用单因素设计,普莱克特-伯曼,最的上升,以及响应表面分析.
- 研究各种介质成分和发酵参数的影响.
主要成果:
- 确定了最优的介质:葡萄糖 (360.61 g/L), (50 g/L),酵母提取物 (14.65 g/L),KH2PO4 (5.49 g/L),MgSO4 (0.40 g/L),CuSO4 (0.01 g/L).这些介质中最优的介质是葡萄糖 (360.61 g/L), (50 g/L),酵母提取物 (14.65 g/L),KH2PO4 (5.49 g/L),MgSO4 (0.40 g/L),CuSO4 (0.01 g/L).
- 确定最佳条件:pH 6.0,1.10%的注射液,12.5毫升/250毫升体积,120rpm,21°C,53.50小时.
- 取得了5.50 × 10^9 CFU/mL的总殖民地数量,增加了31倍.
结论:
- 优化条件显著提高了S. fibuligera Y1402的细胞密度.
- 为S. fibuligera的工业规模生产和应用提供了基础.
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