机械和功能研究对微生物诱导的Wolfiporia cocos液体发酵产品
Zhikang Yang1, Congbao Su1, Zhoujie Xu1
1College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Foods (Basel, Switzerland)
|May 25, 2024
概括
通过液体发酵共同发酵Wolfiporia cocos和Ganoderma lucidum可显著增加菌根生物质和细胞内多糖. 这种增强的提取物显示出改善的抗瘤特性,为功能性食品提供了潜力.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 液体发酵是从食用中提取多糖的有效方法.
- 沃尔菲波里亚可可斯和Ganoderma lucidum以它们的药用特性和多糖含量而闻名.
研究的目的:
- 为了研究共发酵对Wolfiporia cocos.的多糖生产和生物质的影响.
- 为了评估来自共发酵的多糖的生理活动.
- 探索增强型多糖化合物合成背后的分子机制.
主要方法:
- 建立了三个可食用的共发酵系统.
- 量化菌生物质和细胞内多糖含量.
- 提取的多糖的评估生理活动 (抗瘤特性).
- 对Wolfiporia cocos mycelium进行了转录基因分析.
主要成果:
- 这种Wolfiporia cocos-Ganoderma lucidum共发酵系统显著增加了菌生物质57.71% (单独与W. cocos相比) 和91.22% (单独与G. lucidum相比).
- 在共同发酵系统中,细胞内多糖含量显著增加.
- 来自共发酵的多糖体表现出增强的抗瘤细胞增值和抗瘤细胞迁移活动.
- 转录基因分析显示,在对外源物质的反应中,ALG10 (α-glycosyltransferase) 的上调和MAN1B (α-glycosidase) 的下调.
结论:
- 使用液体培养的Wolfiporia cocos和Ganoderma lucidum的共同发酵是增加多糖产量和增强生物活性的一种有希望的策略.
- 这项研究提供了对多糖合成调节的分子见解,识别了关键基因 (ALG10,MAN1B).
- 这些发现支持Wolfiporia cocos和Ganoderma lucidum多糖的发展,作为功能性食品成分和新产品开发.
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