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在卵巢癌中NAD(P) H-氧化降解酶的活性

Maria V Fedorova¹, Vladimir I Voznesensky², Elena A Sosnova³

¹Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, 111123 Moscow, Russia.

|May 25, 2024

概括

反应性氧物种 (ROS) 与癌症有关. 这项研究发现,在晚期卵巢癌中,NADH和NADPH依赖的氧化还原酶 (CYB5R和CYPOR) 的活性增加,与腹液相关.

科学领域:

生物化学生物化学
在瘤学瘤学.
细胞生物学细胞生物学

背景情况:

反应性氧物种 (ROS) 在癌症发展中起着复杂的作用.
微体NADH和NADPH依赖的氧化还原酶是细胞内ROS的关键生产者,但它们在卵巢癌的氧化平衡中的具体参与仍然不清楚.

研究的目的:

为了研究细胞染色体b5减少酶 (CYB5R) 和细胞染色体P450减少酶 (CYPOR) 在卵巢癌组织和腹液中的活性.
评估腹液与卵巢癌进展和分化有关的抗氧化能力.
探索氧化还原酶活性,腹膜液抗氧化能力和化疗耐药性之间的相关性.

主要方法:

开发了一种新的化学发光测定方法,用NADH和NADPH刺激来测量CYB5R和CYPOR活性.
分析了从腹膜液中分离的卵巢癌组织和细胞.
量化了腹液的抗氧化能力.

主要成果:

在中度和差异化卵巢腺癌中,CYB5R和CYPOR活性显著升高,而在差异化卵巢腺癌和囊腺瘤中则明显升高.
耐化疗瘤的CYB5R和CYPOR活性低于非耐药瘤.
腹液的抗氧化能力随着瘤严重程度的增加 (良性<良好分化<中度/差分化),表明晚期癌症的抗氧化剂过量.

结论:

NAD(P) H氧降解酶和腹腔液的抗氧化潜力在卵巢癌生物化学中起着重要作用.
增加的CYB5R和CYPOR活性与晚期瘤分化和腹液抗氧化能力相关.
研究的参数显示了卵巢癌诊断和预后的前景,开发的试验适用于其他组织.

关键词:

抗氧化能力的抗氧化能力.细胞染色体P450减少酶.细胞染色体b5减少酶的作用.卵巢癌是发生在卵巢的癌症.腹腔周围的液体

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