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探索冷等离子体对表皮黑色素发生的影响In Situ和In Vitro

Sybille Hasse¹, Marie-Christine Sommer¹, Sebastian Guenther²

¹Leibniz Institute for Plasma Science and Technology e.V. (INP), a Member of the Leibniz Health Technologies Research Alliance, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.

International journal of molecular sciences

|May 25, 2024

概括

冷大气等离子体 (CAP) 在培养的人类黑色素细胞中没有显著影响黑色素合成. 在皮肤活检中,重复暴露于CAP会增加黑色素,这表明色素失调的风险很小.

科学领域:

皮肤病学皮肤病学
生物物理学的生物物理.
细胞生物学细胞生物学

背景情况:

在表皮黑色素细胞中的黑色素合成决定了皮肤颜色.
反应性氧物种 (ROS) 在调节黑色素产生的过程中起着至关重要的作用.
冷大气等离子体 (CAP) 产生可调节的ROS,促使人们对其对黑色素发生的作用进行研究.

研究的目的:

评估CAP对人类黑色素细胞和皮肤中的黑色素合成的影响.
为了比较CAP的效果与已知的黑色素生成兴奋剂,如IBMX和紫外线辐射.
评估CAP在治疗色素障碍方面的潜力及其对有害超色素的风险.

主要方法:

在CAP应用中使用了气驱动的等离子喷射器kINPen® MED.
在体外 (培养的人类黑色素细胞) 和现场 (全厚人体皮肤活检) 分析黑色素含量.
使用组织化学,NIR自光流细胞计和HPLC-MS进行黑色素量化和分化.

主要成果:

重复暴露于CAP导致人类皮肤活检中的黑色素含量增加.
与紫外线辐射和IBMX相比,培养的人类黑色素细胞对CAP仅表现出轻微的反应.
通过HPLC-MS分析,可以区分eumelanin和pheomelanin.

结论:

CAP不太可能有效治疗皮肤色素障碍.
作为CAP在皮肤学应用中的不良影响,增色的风险可能是可以忽略不计的.

关键词:

皮肤上的黑色素细胞黑色素的产生是黑色素的产生.流体流体流体血流体血医学血医学

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