调节生物膜在FZB42中分散的细菌
Lin Shao1,2, Zizhu Shen2, Meiju Li1
1Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry and Grass, Nanjing Forestry University, Nanjing 210037, China.
International journal of molecular sciences
|May 25, 2024
概括
细菌 velezensis 中的未注释的基因ccdC促进生物膜分散,使细菌细胞能够逃脱成熟的生物膜并殖民新的区域. 这一过程与周期性迪加尼酸盐 (c-di-GMP) 水平的调节有关.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 植物科学 植物科学
背景情况:
- 百菌 velezensis FZB42是一种促进植物生长的草原细菌 (PGPR),对于通过生物膜对草原圈殖民至关重要.
- 了解生物膜分散,细胞逃脱成熟生物膜的最后阶段,对于细菌的传播和殖民至关重要.
- 在PGPR中控制生物膜分散的调节机制在很大程度上仍然未被描述.
研究的目的:
- 调查未注释的基因ccdC在Bacillus velezensis FZB42.2.的生物膜分散中的作用.
- 阐明ccdC影响细菌运动和生物膜逃逸的机制.
- 探讨ccdC,循环 diguanylate (c-di-GMP) 水平和生物膜调节之间的联系.
主要方法:
- 构建和分析一个缺乏ccdC的Bacillus velezensis菌株.
- 使用液气接口培养物进行生物膜形成和分散试验.
- 在野生型和突变菌株中测量细胞内周期性迪加尼酸盐 (c-di-GMP) 水平.
主要成果:
- 缺乏ccdC的菌株表现出生物膜分散受损,形成纹的皮质,没有活跃的细胞逃逸.
- 分散,依赖于运动性,受到ccdC基因的存在和功能的积极影响.
- 与野生型菌株相比,ccdC缺乏的菌株显示出明显更高的c-di-GMP水平.
结论:
- 没有注释的基因ccdC在调节Bacillus velezensis中的生物膜分散方面发挥着至关重要的作用.
- B. velezensis似乎利用一种c-di-GMP依赖的生物膜分散机制,类似于Pseudomonas aeruginosa.
- ccdC通过调节c-di-GMP水平来影响生物膜扩散,影响细菌运动和殖民.
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