IQGAP1 调节了动氨酸聚合,并有助于白色素诱导的肺纤维化
Tanjina Akter1, Ilia Atanelishvili1, Richard M Silver1
1Department of Medicine, Medical University of South Carolina, 96 Jonathan Lucas Street, Suite 912, Charleston, SC 29425, USA.
International journal of molecular sciences
|May 25, 2024
概括
在小鼠中,含有GTPase激活蛋白 (IQGAP1) 的智商动机缺失降低了肺纤维细胞收缩活性和白素诱导的肺纤维化. 这表明IQGAP1是肺纤维化抗纤维治疗的潜在标.
科学领域:
- 肺部医学 肺部医学
- 细胞生物学 细胞生物学
- 纤维化研究 纤维化研究
背景情况:
- 含有GTPase激活蛋白 (IQGAP1) 的智商动机在结核病相关的间歇性肺病 (SSc-ILD) 肺纤维细胞 (LF) 中升高.
- IQGAP1调节LF中的α-平滑肌肉动蛋白 (SMA) 表达和组织,有助于SSc.
- IQGAP1在肺纤维化发展中的作用需要进一步研究.
研究的目的:
- 为了比较肺纤维化在存在和不存在IQGAP的发展1.
- 为了研究IQGAP1在白血素诱导的肺纤维化中的功能作用.
主要方法:
- 在IQGAP1淘汰赛 (KO) 和野生类型 (WT) 小鼠中使用布莱米辛灌注诱导肺纤维化.
- 分析了肺组织的actin聚合,纤维细胞收缩活性,以及SMA,TGFβ和原蛋白的表达.
- 评估了炎症细胞数量和促炎性细胞因子表达.
主要成果:
- 与WT小鼠相比,IQGAP1 KO小鼠显示了减少的动因聚合和肺动因积累.
- 在暴露于白胺后,IQGAP1 KO小鼠表现出下降的LF收缩活性,减少的SMA,TGFβ和原表达,以及较低的纤维化得分.
- 在KO和WT小鼠之间没有观察到炎症细胞数量或促炎细胞因子表达的显著差异.
结论:
- IQGAP1在白血素诱导的肺纤维化发展中起着重要作用.
- 缺少IQGAP1会减弱肺纤维细胞收缩活性,并减少肺纤维化.
- 在肺纤维化中,IQGAP1代表了抗纤维治疗的潜在治疗标.
关键词:
含有GTPase激活蛋白 (IQGAP1) 的智商模式布莱莫辛 (Bleomycin) 是一种可怕的药物.一个淘汰赛鼠标.肺部纤维细胞是什么硬化症 (全身性硬化症,SSc) 相关的间歇性肺病 (ILD).α-平滑肌肉行为蛋白 (SMA)更多相关视频
02:46A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization
Published on: January 20, 2023
3.5K
07:38A Multimodal Imaging Approach Based on Micro-CT and Fluorescence Molecular Tomography for Longitudinal Assessment of Bleomycin-Induced Lung Fibrosis in Mice
Published on: April 13, 2018
11.5K
相关概念视频
01:19Actin Filament Depolymerization
3.1K
Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
In F-actin, the ADF/cofilin proteins...
3.1K
01:13Actin Polymerization and Cell Motility
5.2K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.2K
01:14Generation of Straight or Branched Actin Filaments
2.9K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
2.9K
01:26Introduction to Actin
5.1K
Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution. Actin coding genes are conserved within species and across...
5.1K
01:42Actin Polymerization
6.5K
Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶ nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
6.5K
01:39Mechanism of Filopodia Formation
2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K