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与衰老相关的变化发生在介质细胞干细胞的母体体内

Diana Matveeva¹, Daria Kashirina¹, Mariia Ezdakova¹

¹Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye Shosse, 76a, 123007 Moscow, Russia.

International journal of molecular sciences

|May 25, 2024

概括

衰老会改变介质干细胞 (MSC),改变它们的细胞外基质 (ECM),并促进组织损伤. 衰老的MSC重塑其ECM,通过改变蛋白质生产和炎症信号对周围细胞产生负面影响.

科学领域:

老年学是一门学科.
细胞生物学细胞生物学
生物化学生物化学

背景情况:

衰老与组织和细胞的变化有关,衰老细胞在微环境中发挥着关键作用.
介质干细胞 (MSC) 对于组织平衡至关重要,但其细胞外基质 (ECM) 中与年龄相关的变化仍未得到充分研究.
MSC ECM调节邻近细胞的活动,使其与年龄相关的变化对理解组织利基衰老至关重要.

研究的目的:

研究老化中介干细胞 (MSCs) 的母体体 (ECM组件) 的与年龄相关的变化.
分析老化MSCs的ECM,条件介质 (CM) 和细胞溶解物的蛋白质组概况.
了解衰老的MSC母体如何影响组织微环境.

主要方法:

对老化MSC的分离部分 (ECM,CM,细胞溶解物) 的广泛蛋白质组分析.
在老年人与年轻的MSC中量化和比较蛋白质水平.
识别参与结构,调节,炎症和前期衰老功能的关键蛋白质.

主要成果:

结构性ECM蛋白质 (原体,纤维素,纤维素) 和细胞保护因子 (甲素,甲素,S100蛋白) 的显著下调.
炎症性蛋白质和益纤维素通路调节者的水平增加.
在衰老的MSC母体体中对前衰老蛋白 (SERPINE1,THBS1,GDF15) 的升级调节.

结论:

衰老的MSC表现出改造后的ECM,其特点是结构完整性和保护功能降低.
改变的MSC母体和细胞因子信号一起,有助于形成一种亲炎性和亲纤维性组织微环境.
衰老的MSC通过其修饰的ECM对邻近细胞产生负面影响,加剧组织衰老和功能障碍.

关键词:

细胞外矩阵 (ECM) 是一个细胞外矩阵.一个母体组的母体组.中介细胞干细胞 (MSCs)衰老是一种老化.与衰老相关的分泌表现型 (SASP)

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