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通过下一代测序对B型肝炎病毒进行基因定型:混合感染的检测和测序保存分析

Eva Dopico^1,2, Marta Vila³, David Tabernero^3,4,5

¹Department of Microbiology, Metropolitana Sud Territorial Clinical Laboratory, Bellvitge University Hospital, Institut Català de la Salut (ICS), 08907 Hospitalet de Llobregat, Spain.

International journal of molecular sciences

|May 25, 2024

概括

一种新的下一代测序 (NGS) 方法可以准确地确定乙型肝炎病毒 (HBV) 的基因型,并检测混合物. 在HBV 5' X基因中,两个保存区域被确定为抗病毒疗法的潜在目标.

科学领域:

病毒学病毒学
遗传学遗传学是一个
分子生物学分子生物学

背景情况:

乙型肝炎病毒 (HBV) 感染是一个全球性的健康问题.
准确的HBV基因型定型对于了解传播模式和指导治疗至关重要.
目前的基因型鉴定方法可能缺乏检测混合感染和多种基因型的灵敏度.

研究的目的:

开发一种高度敏感的下一代测序 (NGS) 方法来确定HBV的基因型.
为了检测HBV基因型混合和基因间重组.
为了确定HBV基因型中的保存区域,以进行潜在的治疗向.

主要方法:

使用NGS对HBV X基因 (5X) 区域的preS和5'端进行分析.
使用基于距离的歧视方法 (DB规则) 用参考序列 (基因型A-H) 来对1852个单元型的基因型定型.
使用信息内容分析评估HBV基因型 (A,C,D,E) 之间的序列保存.

主要成果:

基于NGS的方法在125个临床样本中成功确定了HBV基因型.
在22.40%的病例中发现了基因型混合,在23.20%的病例中发现了基因型间重组.
在评估的基因型中,在5X单元型中确定了两个保存区域,而在preS单元型中显示了有限的保存.

结论:

使用DB规则开发了一种基于NGS的准确而敏感的HBV基因型定型方法.
已识别的保存的5X区域代表了基于RNA干扰的抗病毒疗法的有希望的目标.
该方法有助于检测HBV基因型混合物和重组事件.

关键词:

基于距离的歧视方法.这是一种RNA干扰.保护保护保护基因型基因型基因型乙型肝炎X基因乙型肝炎X基因乙型肝炎病毒的感染.信息内容信息内容信息内容这是下一代测序.在此之前,我们已经做了预SS.准物种类型准物种类型

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