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鉴定和分析与小麦粒发育相关的lncRNA和circRNA

Meng Wang¹, Lu Wang¹, Shuanghong Wang¹

¹College of Agronomy, Anhui Agricultural University, Hefei 230036, China.

International journal of molecular sciences

|May 25, 2024

概括

这项研究揭示了复杂的调节网络,涉及小麦粒发育中的长非编码RNA (lncRNA) 和圆形RNA (circRNA). 这些相互作用,特别是由特定的microRNAs (miRNAs) 介导的相互作用,对于谷物的重量和形状形成至关重要.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
农业科学农业科学

背景情况:

长非编码RNAs (lncRNAs) 和循环RNAs (circRNAs) 在小麦粒发育中的作用在很大程度上仍未被描述.
了解这些监管要素对于提高小麦产量和质量至关重要.

研究的目的:

在小麦粒发育过程中对lncRNAs和circRNAs进行表征.
构建涉及lncRNAs,circRNAs和microRNAs (miRNAs) 的调节网络.
确定影响小麦谷物发展的关键监管模块.

主要方法:

两种小麦品种的全转录组测序,在授粉后的10,20和30天具有不同的谷物重量.
对lncRNAs和circRNAs的差异表达分析.
构建 lncRNA-miRNA 和 circRNA-miRNA 相互作用网络.
使用定量实时PCR (qRT-PCR) 和双露西法酶记者分析进行验证.

主要成果:

在两个小麦品种之间,在不同的发育阶段,发现了大量差异表达的lncRNAs和circRNAs.
circRNAs表现出比lncRNAs更复杂的相互作用网络,涉及诸如tae-miR1177,tae-miR1128和tae-miR1130b-3p.p.等关键miRNAs.
确定TaNF-YB1和TaTGW-7B是特定miRNA准的关键调节基因,他们的调节关系经过实验证实.

结论:

在调节小麦粒的发育和形状方面,circRNAs比lncRNAs发挥更广泛的作用.
一个circRNA-miRNA-基因调节模块被建议对小麦谷物发育至关重要.
这项研究为非编码RNA介导的谷物谷粒发育调节提供了新的见解.

关键词:

环RNA 环RNA 是一个环RNA.在ncRNA中,我们可以这是一个小RNARNA.调节性基因调节性基因小麦的谷物小麦的谷物

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