相关实验视频

Updated: Jun 25, 2025

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Published on: October 25, 2011

跨瘤类型的ROR1蛋白表达的异质概况

Maria Gabriela Raso¹, Elizve Barrientos Toro¹, Kurt Evans²

¹Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

|May 25, 2024

概括

ROR1蛋白在间皮瘤和其他一些癌症中表达高,呈现出新的治疗点. 这项研究分析了许多瘤类型的ROR1表达,以指导ROR1向的癌症疗法.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症治疗方法癌症治疗方法

背景情况:

Wnt受体ROR1是一个有前途的癌症治疗标.
ROR1组织表达模式尚未得到充分理解.
了解ROR1表达对于开发向疗法至关重要.

研究的目的:

为了研究ROR1蛋白表达在广泛的人类瘤类型.
为了确定具有高ROR1表达的特定癌症,用于潜在的向治疗.
为了评估患者衍生异种移植 (PDXs) 中的ROR1表达.

主要方法:

对ROR1蛋白表达的免疫组织化学分析.
对12个多瘤组织微阵列 (TMAs) 和5个瘤TMAs的评估.
分析了来自19种不同的组织学类型的6个PDX TMA,共计1142例患者病例和140例PDX.

主要成果:

在不同瘤类型中,ROR1表达异质.
在中皮瘤 (84.6%),脂肉瘤 (36.1%),GIST (33.3%) 和子宫内膜腺癌 (28.9%) 中观察到高ROR1患病率.
低ROR1表达 (<13%) 在乳腺,肺,,肝脏,泌尿器,结肠癌,质母细胞瘤,胆管癌和乳腺髓瘤中发现. 没有表达在介质细胞胆固醇瘤,狂宫肌肉瘤或胃腺癌.

结论:

间皮瘤表现出频繁且高的ROR1表达,表明了新的治疗机会.
选择性瘤如胃食道GIST显示高ROR1表达,这表明ROR1预选是有益的.
这些发现支持针对特定癌症指标开发ROR1向疗法.

关键词:

IHC 蛋白质表达的表达方式在ROR1中,ROR1是指ROR1.组织表达的组织表达.类似于氨酸激酶的孤儿受体.

更多相关视频

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

Published on: January 22, 2013

相关概念视频

The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...