在山西老中分离和检测catechins和epicatechins,使用固相提取和与HPLC相结合的疏水性深性溶剂
Baoqing Bai1,2, Dan Shen1, Siyuan Meng1
1School of Life Science, Shanxi University, Taiyuan 030006, China.
Molecules (Basel, Switzerland)
|May 25, 2024
概括
一种新的固体相提取和深度环氧化溶剂方法 (SPE-DES-HPLC) 能够有效量化老化中的甲基素和基素. 这种环保的方法为醇分析提供了切实可行的替代方案.
科学领域:
- 分析化学 分析化学
- 绿色化学 绿色化学
背景情况:
- 山西老 (SAV) 含有有价值的黄化合物,如甲素和甲素.
- 准确量化这些化合物对于质量评估至关重要.
- 传统的方法可能缺乏效率或环保性.
研究的目的:
- 开发和优化一种新的,环保的,快速的方法来提取和量化SAV中的catechin和epicatechin.
- 验证方法的性能并评估其对环境的影响.
主要方法:
- 结合固体相提取 (SPE) 与疏水性深溶解剂 (DES) 和高性能液体色谱 (HPLC) 的组合.
- 优化参数,包括化溶剂,树脂剂量,DES比率和提取时间,使用单向实验和Box-Behnken方法.
- 通过确定系数,精度和回收率验证方法的有效性.
主要成果:
- 确定了疏水性DES (四乙和八酸) 比率 (1:3),XAD-2树脂剂量 (188毫克) 和吸附时间 (11分钟) 的最佳条件.
- 方法表现高,确定系数≥0.9917,精度<5%,回收率在98.8%至118.8%之间.
- 使用分析生态规模和AGREE进行的环境评估证实了该方法的环保性质.
结论:
- 开发的SPE-DES-HPLC方法是一种实用,快速和环保的技术,用于分析SAV中的黄.
- 这种创新方法为评估老样本中的黄含量提供了可靠的基础.
- 这项研究突出了深度环氧溶剂在绿色分析化学中的潜力.
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