乳杆菌后生物体对Streptococcus mutans生物膜的化学表征和影响
Guilherme Bandeira Santana1, Patrick Veras Quelemes1, Enedina Rodrigues da Silva Neta2
1Postgraduate Program in Dentistry, Federal University of Piauí, Teresina 64049-550, Brazil.
Microorganisms
|May 25, 2024
概括
来自Lacticaseibacillus rhamnosus LR-32的后生物抑制了Streptococcus mutans的生长和生物膜的形成. 这项研究在化学上对后生物特征进行了鉴定,并证明了其在减少生物膜中可行的细菌和关键基因表达方面的有效性.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 后生物质是益生菌的可溶性因子或代谢产物.
- 突变性链球菌 (Streptococcus mutans) 是牙腐烂的一个关键病原体,形成生物膜.
- 了解后生物组合和功能对于开发新型治疗策略至关重要.
研究的目的:
- 为了化学描述Lacticaseibacillus rhamnosus LR-32.2. 的后生物.
- 评估这种后生菌对Streptococcus mutans浮游生物生长和生物膜发育的体外影响.
主要方法:
- 通过离心和过L. rhamnosus LR-32培养的后生物提取.
- 使用气体染色体质谱法 (GC-MS) 进行化学表征.
- 在体外测试评估S. mutans浮游生物生长抑制,生物膜活性的细菌数量,干重和基因表达 (葡萄糖转移酶,VicR).
主要成果:
- 17种化合物,包括糖,氨基酸,维生素和酸在后生物体中被确定.
- 后生物抑制了浮游生物S. mutans的生长.
- 在S. mutans生物膜中观察到可活性的细菌显著减少,干重和GTfB,GTfC,GTfD和VicR基因表达的下调 (p <0.05).
结论:
- L. rhamnosus LR-32 的后生物对S. mutans. 具有显著的抗生物膜特性.
- 这种特征性的后生物质通过抑制生长和调节毒性基因表达,有效地损害了S. mutans生物膜的发展.
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