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在捷克农场Corynebacterium pseudotuberculosis的绵羊和山羊菌株-一项比较研究

Jirina Markova¹, Denisa Langova¹, Vladimir Babak¹

¹Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, 62100 Brno, Czech Republic.

|May 25, 2024

概括

小反动物的病变性淋巴腺炎 (CLA) 是由Corynebacterium伪结核病引起的. 基因组分析显示,在多种菌株中保留了毒性因子,这表明虫生物群中的克隆行为.

科学领域:

兽医微生物学兽医微生物学
基因组学就是基因组学.
传染性疾病传染性疾病

背景情况:

乳腺淋巴炎 (Caseous lymphadenitis,简称CLA) 是一种影响绵羊和山羊的全球性疾病.
它是由可选择性细胞内病原体Corynebacterium pseudotuberculosis引起的.
了解病原体的毒性和遗传构成对于疾病控制至关重要.

研究的目的:

为了表征导致CLA的Corynebacterium伪结核病的捷克分离物.
为了研究C. pseudotuberculosis中毒性因子的基因组基础.
将捷克菌株与全球隔离品进行比较,并确定它们的生物和遗传相关性.

主要方法:

对来自捷克绵羊和山羊的33 C. 伪结核菌株进行隔离和抗生素敏感性测试.
评估生物膜的形成和消毒剂的耐药性.
全基因组测序和对比基因组分析,使用123个公开的C. pseudotuberculosis基因组.
鉴定毒性因子的遗传决定因素,包括粘附性,铁吸收,脂酶D,外o-α-sialidase和CP40蛋白.

主要成果:

所有33个捷克分离物都被确定为Corynebacterium pseudotuberculosis biovar ovis. 这些分离物都是Corynebacterium pseudotuberculosis的生物.
关键毒性因子的遗传决定因素 (粘附性,铁吸收,脂酶D,外o-α-sialidase,CP40) 在所有分析的基因组中都存在.
对比基因组学揭示了全球生物虫菌株之间的高度相似性,这表明了克隆进化.
捷克菌株对测试的抗生素表现出敏感性,但也表现出形成生物膜的能力和对消毒剂的耐药性.

结论:

导致CLA的捷克菌株Corynebacterium pseudotuberculosis属于biovar ovis,并表现出保存的毒性基因配置文件.
生物鸟菌株之间的高基因组相似性强调了它们的克隆性质和广泛传播的潜力.
对生物膜形成和消毒剂耐药性机制的进一步研究是有效的CLA管理的必要条件.

关键词:

CAZymes 的意思是这就是CLA.科里尼细菌的伪结核病 (Corynebacterium pseudotuberculosis) 已经成为一种疾病.抗生素敏感性抗生素敏感性进行比较的基因组学.消毒剂是一种消毒剂.小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的毒性因素是毒性的因素.

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