在一个人性化的微生物群小鼠模型中,探索用trimethoprim-sulfamethoxazole和dexamethasone对肠道微生物群的改变
George B H Green1, Alexis N Cox-Holmes1, Olivia Backan1,2
1Department of Cell, Developmental and Integrative Biology, Birmingham, AL 35294, USA.
Microorganisms
|May 25, 2024
概括
常见的质母细胞瘤药物trimethoprim-sulfamethoxazole (TMP-SMX) 和dexamethasone显著改变了敏感人性化小鼠的肠道微生物组. 这些变化可能会影响癌症治疗的有效性.
科学领域:
- 微生物学 微生物学
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 质母细胞瘤的治疗通常涉及支持药物,如trimethoprim-sulfamethoxazole (TMP-SMX) 用于预防感染和德克萨米用于脑.
- 肠道微生物组在调节癌症治疗疗效方面发挥着至关重要的作用.
- 了解药物对肠道微生物群的影响对于优化质母细胞瘤患者的治疗结果至关重要.
研究的目的:
- 调查TMP-SMX和德克萨米他对肠道微生物组合和功能的影响.
- 为了确定不同的人性化微生物组合是否对这些药物表现出不同的敏感性.
主要方法:
- 人性化微生物群 (HuM) Rag1-/-小鼠 (HuM1Rag和HuM2Rag) 用TMP-SMX或德克萨梅他通过口腔 gavage治疗了一周.
- 在治疗前,治疗后1周和治疗后3周收集了便样本.
- 用16S rRNA测序分析细菌DNA,以评估微生物组的变化.
主要成果:
- 在HuM1Rag小鼠中,TMP-SMX治疗诱导了α多样性,β多样性和功能途径的显著改变,对HuM2Rag小鼠的影响最小.
- 德克萨米他还在HuM1Rag小鼠中引起了显著的微生物组变化,而HuM2Rag小鼠在很大程度上没有受到影响.
- 微生物群对这些药物的敏感性在两种小鼠模型之间有所不同.
结论:
- 标准的质母细胞瘤药物可以扰乱肠道微生物群.
- 干扰的程度取决于个体的微生物组合.
- 这些药物诱导的微生物组变化可能会影响质母细胞瘤治疗的疗效.
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