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在Bemisia相关的Dicistrovirus 2中,因子依赖的内部核糖体进入点和-1编程的移信号

Yihang Chen¹, Subash Chapagain¹, Jodi Chien¹

¹Department of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.

|May 25, 2024

概括

这项研究揭示了一种新型因子依赖的内部核糖体进入部位 (IRES) 机制,以及白相关的二病毒2 (BaDV-2) 中的-1移信号,扩大了我们对病毒RNA策略的理解.

科学领域:

分子生物学分子生物学
病毒学病毒学
RNA结构和功能 RNA结构和功能

背景情况:

双基因病毒间基因 (IGR) 内部核糖体进入点 (IRES) 通常采用简化,因子独立的翻译启动机制.
这些IRES通常具有两到三个重叠的伪结,与核糖体子单元相互作用.
之前的一项研究预测了白贝米西亚相关的二分流病毒2 (BaDV-2) 中非典型的IGR IRES和-1编程移 (-1FS) 信号.

研究的目的:

为了研究预测的BaDV-2 -1 FS信号和IGR IRES的功能特征.
阐明BaDV-2使用的翻译启动机制.
描述参与BaDV-2翻译调节的RNA结构元素.

主要方法:

在体外使用双声记者来评估BaDV-2 -1 FS信号的活性和IGR IRES.
使用删除和突变分析来映射IRES元素并确定关键结构特征.
研究了核糖体结合和对翻译启动因子抑制剂 (eIF2,eIF4A) 的敏感性.

主要成果:

BaDV-2 -1 FS信号在体外驱动移,通过滑动序列和下游茎环介导.
BaDV-2 IGR IRES支持从AUG编码器启动翻译,但需要宿主因素,与典型的dcistrovirus IRES不同.
BaDV-2 IRES对eIF2和eIF4A抑制剂敏感,并且在启动过程中看起来没有结构或采用动态构造.

结论:

在dcistrovirus家族中展示了第一个-1FS移信号.
在dcistrovirus中发现了一个新的,因子依赖的IRES机制,挑战了以前的模型.
突出了病毒RNA结构用于调节蛋白质合成的多样化策略.

关键词:

这里是IRES IRES.这是一个RNARNARNARNARNA.框架转移的框架转移.病毒病毒病毒病毒

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