Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Viral Recombination00:57

Viral Recombination

23.4K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.4K
Sampling Plans01:23

Sampling Plans

180
Sampling is a crucial step in analytical chemistry, allowing researchers to collect representative data from a large population. Common sampling methods include random, judgmental, systematic, stratified, and cluster sampling.
Random sampling is a method where each member of the population has an equal chance of being selected for the sample. It involves selecting individuals randomly, often using random number generators or lottery-type methods. For example, when analyzing the properties of a...
180

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Peaked-to-flat transition in quasispecies structure evolution.

Virus evolution·2026
Same author

Unveiling pathogens and contaminants: refining metagenomics for clinical diagnostics.

Frontiers in microbiology·2026
Same author

Deep Sequencing Analysis of Hepatitis C Virus Subtypes and Resistance-Associated Substitutions in Genotype 4 Patients Resistant to Direct-Acting Antiviral (DAA) Treatment in Egypt.

International journal of molecular sciences·2025
Same author

Viral Quasispecies Inference from Single Observations-Mutagens as Accelerators of Quasispecies Evolution.

Microorganisms·2025
Same author

Deep Sequencing Reveals Dual Evolution of SARS-CoV-2: Insights Into Defective Genomes From Wuhan-Hu-1 Variants to Omicron Subvariants.

Journal of medical virology·2025
Same author

Association of Liver Damage and Quasispecies Maturity in Chronic HCV Patients: The Fate of a Quasispecies.

Microorganisms·2024

相关实验视频

Updated: Jun 25, 2025

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

29.6K

病毒准物种稀疏化:用或不用替换进行亚样本?

Josep Gregori1, Marta Ibañez-Lligoña1,2,3, Sergi Colomer-Castell1,2,3

  • 1Liver Diseases-Viral Hepatitis, Liver Unit, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron Hospital Universitari, Vall d'Hebron Barcelona Hospital Campus, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.

Viruses
|May 25, 2024
PubMed
概括
此摘要是机器生成的。

样本大小规范化对于在下一代测序 (NGS) 数据中对准物种多样性进行比较至关重要. 稀疏化,特别是没有替换的重新抽样,确保准确的比较,特别是对于罕见的单元型.

关键词:
简单类型 简单类型的类型转基因组学是指转基因组学.准物种类型 准物种类型稀有化是一种稀有化.部分采样采样

更多相关视频

Estimating Virus Production Rates in Aquatic Systems
10:49

Estimating Virus Production Rates in Aquatic Systems

Published on: September 22, 2010

12.7K
Following the Dynamics of Structural Variants in Experimentally Evolved Populations
04:52

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

957

相关实验视频

Last Updated: Jun 25, 2025

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

29.6K
Estimating Virus Production Rates in Aquatic Systems
10:49

Estimating Virus Production Rates in Aquatic Systems

Published on: September 22, 2010

12.7K
Following the Dynamics of Structural Variants in Experimentally Evolved Populations
04:52

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

957

科学领域:

  • 病毒学 病毒学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 在准物种多样性研究中,比较不同大小的样本至关重要.
  • 多样性指数可能对样本大小变化敏感,使比较复杂化.
  • 下一代测序 (NGS) 产生了需要规范化的大型数据集.

研究的目的:

  • 突出样本大小规范化在准物种多样性分析中的重要性.
  • 检查不同的重新抽样方案,以使NGS数据正常化.
  • 了解规范化对多样性指数的影响.

主要方法:

  • 利用稀疏化作为一个规范化工具.
  • 采用重新采样而没有替代正常化,特别是对于罕见的单元类型.
  • 分析了具有不同基因组合的假设类物种.

主要成果:

  • 证明了样本大小规范化对于准确的准物种多样性比较的必要性.
  • 展示了重复重新抽样的有效性,而没有取代高覆盖率的NGS数据.
  • 确定不同的多样性指数对样本大小有不同的敏感性.

结论:

  • 用NGS数据进行可靠的准物种多样性研究,必须对样本大小进行规范化.
  • 建议在没有替代的情况下进行重新采样,以实现正常化,当罕见的单元类型感兴趣时.
  • 某些多样性指数可能允许直接比较或安全重新采样与替换.