对感染人类乳腺瘤病毒 (HPV) 的乳腺系细胞的免疫反应
Daffany Luana Santos1, Bianca de França São Marcos1, Georon Ferreira de Sousa2,3
1Laboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária, Recife 50670-901, Pernambuco, Brazil.
人类乳头瘤病毒 (HPV) 基因,特别是E6和E7,被发现可以抑制乳腺癌细胞中的免疫反应. 这些病毒性瘤基因降低了淋巴细胞和单细胞的功能,可能会阻碍抗瘤免疫力.
科学领域:
- 在瘤学瘤学.
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 乳腺癌是全球领先的瘤,病毒感染如人类乳腺瘤病毒 (HPV) 涉及致癌.
- 尽管已知HPV感染会调节宿主的免疫反应,从而导致免疫抑制,但HPV在乳腺癌中的作用仍在争论中.
研究的目的:
- 研究HPV瘤基因 (E5,E6,E7) 对乳腺癌细胞系 (MDA-MB-231) 免疫细胞功能的特定影响.
主要方法:
- 感染MDA-MB-231细胞的HPV E5,E6和E7瘤基因.
- 感染细胞与外周血液单核细胞共同培养.
- 免疫类型鉴定用于评估免疫调节,包括细胞因子概况和细胞活性.
主要成果:
- 感染过的细胞显示CD4+T细胞增加,特别是调节性T细胞 (Tregs).
- 促炎性细胞因子 (IFN-γ,TNF-α,IL-17) 减少,CD8+和CD56+淋巴细胞细胞毒性活性降低,特别是在E6和E7瘤基因.
- E6和E7瘤基因降低了单细胞表达和M1配置激活,而观察到的单细胞表现出促炎性细胞因子释放.
结论:
- HPV 瘤基因,特别是 E6 和 E7,可以降低 MDA-MB-231 乳腺癌细胞系中淋巴细胞和单细胞的数量和功能.
- 这表明HPV可能有助于乳腺癌免疫逃避的机制.
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