相关实验视频

Updated: Jun 25, 2025

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Published on: April 3, 2018

CRISPR屏幕显示PACT是登革热病毒复制的亲病毒因素

Shwetha Shivaprasad^1,2, Wenjie Qiao¹, Kuo-Feng Weng¹

¹Department of Microbiology & Immunology, Stanford University SOM, Stanford, CA 94305, USA.

|May 25, 2024

概括

研究人员发现,干扰素诱导蛋白激酶 (PACT) 的蛋白激活剂令人惊地促进了登革热病毒 (DENV) 复制. 这一发现为开发针对宿主的抗病毒疗法来治疗DENV感染提供了新的途径.

科学领域:

病毒学病毒学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

登革热病毒 (DENV) 感染全球数百万人,目前没有抗病毒药物.
克里斯普尔查识别了对病毒感染和潜在治疗点至关重要的宿主因素.

研究的目的:

通过CRISPR查识别DENV感染所必需的宿主因素.
阐明蛋白激酶 (PACT) 的蛋白激活剂在DENV复制中的作用.

主要方法:

聚焦的CRISPR图书馆屏幕在人类Huh7细胞中.
在PACT淘汰和再表达后分析DENVRNA和蛋白质水平.
全球基因表达分析以确定受影响的途径.

主要成果:

PACT被确定为DENV.的新型亲病毒因素.
PACT淘汰赛显著降低了DENVRNA和蛋白质水平.
在DENV感染细胞中,PACT淘汰阻止了特定ER相关的亲病毒基因的上调.

结论:

PACT在DENV感染中起着亲病毒作用,促进病毒RNA复制.
PACT可以通过ER相关的亲病毒基因表达来调节DENV复制.
PACT代表了宿主导登革热抗病毒策略的潜在目标.

关键词:

这是一个CRISPR屏幕.登革热病毒感染者宿主病毒的相互作用

更多相关视频

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

Published on: October 30, 2021

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...