在SARS-CoV-2血液至大脑转细胞瘤中调节细胞透性
Taylor E Martinez1,2, Karthick Mayilsamy1,2, Shyam S Mohapatra2,3
1Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Viruses
|May 25, 2024
概括
严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 可以通过穿越血脑屏障 (BBB) 感染大脑. 这项研究揭示了SARS-CoV-2使用特定的内细胞突变途径穿越BBB,感染脑细胞.
科学领域:
- 神经科学是一个神经科学.
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 感染多个器官,包括大脑,但血脑屏障 (BBB) 穿越的机制尚未完全理解.
- 血管酶转化酶2 (ACE2) 和二乙酶4 (DPP4) 在SARS-CoV-2进入大脑中的作用需要进一步调查.
研究的目的:
- 研究SARS-CoV-2穿越BBB的机制.
- 确定ACE2和DPP4受体在BBB细胞组件SARS-CoV-2感染中的参与.
- 评估直接的SARS-CoV-2感染对BBB透性的影响.
主要方法:
- 利用一个由人类静脉内皮细胞 (HUVECs),星球细胞和细胞周细胞组成的跨BBB模型.
- 研究了SARS-CoV-2与ACE2和DPP4受体在BBB细胞组件上的相互作用.
- 分析了病毒进入途径,包括克拉特林和卡韦林介导的内细胞分裂.
- 评估了BBB模型感染后对细胞透性的变化.
主要成果:
- 在BBB模型中,直接感染SARS-CoV-2并没有改变细胞透性.
- 在SARS-CoV-2中,使用了克拉斯林和卡韦林介导的内细胞分裂来穿越BBB.
- 病毒进入导致BBB模型的大脑侧感染,内皮细胞感染最小.
- 无论是ACE2和DPP4受体都涉及到内皮细胞,星细胞和周细胞的感染.
结论:
- BBB易受SARS-CoV-2感染,通过直接的细胞感染和细胞转移.
- SARS-CoV-2 可以通过 ACE2 和/或 DPP4 感染 BBB 的内皮细胞,星体细胞和周细胞.
- SARS-CoV-2的血液到大脑转细胞化可以独立于宿主细胞受体发生.
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