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在非人类灵长类动物中,psoralen无活化SARS-CoV-2疫苗的免疫性和保护效率

John W Sanders¹, Daniel Ewing², Appavu K Sundaram²

¹Section on Infectious Diseases, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.

|May 25, 2024

概括

一种带有辅助剂的新型无活化全病毒COVID-19疫苗 (SARS-CoV-2 PsIV) 显示出对变种的潜力. 在DNA疫苗接种后用作增强剂时,它显著增强了中和抗体,并清除了非人类灵长类动物的感染.

科学领域:

病毒学病毒学
疫苗学疫苗学疫苗学
免疫学免疫学免疫学

背景情况:

严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 变种构成了全球健康挑战,导致疫苗免疫逃避.
目前针对尖端蛋白的受体结合域 (RBD) 的疫苗面临着新出现的突变带来的挑战.
无活化全病毒疫苗提供了一种针对多个病毒抗原的更广泛免疫反应的策略.

研究的目的:

开发和评估一种用Advax-CpG添加辅助剂的psoralen失活的SARS-CoV-2疫苗 (SARS-CoV-2 PsIV).
评估SARS-CoV-2 PsIV在非人类灵长类动物 (NHPs) 的免疫性和疗效.
调查SARS-CoV-2 PsIV作为核酸 (NA) 疫苗的助推疫苗的潜力.

主要方法:

准备一个用psoralen无活化的SARS-CoV-2疫苗 (SARS-CoV-2 PsIV).
用Advax-CpG作为NHPs辅助剂的疫苗的使用.
使用血清微中和试验对抗SARS-CoV-2菌株和变异的中和抗体反应的评估.
在与SARS-CoV-2Delta变种进行鼻腔挑战后,评估疫苗的疗效.

主要成果:

与Advax-CpG辅助的SARS-CoV-2 PsIV诱导了NHPs对华盛顿菌株和三角洲变种的剂量依赖的中和抗体反应.
在DNA接种疫苗后用SARS-CoV-2 PsIV增强的NHP显示出最高的中和抗体水平.
接种DNA疫苗后接种SARS-CoV-2PsIV增剂的动物在挑战后迅速清除了SARS-CoV-2Delta变种感染.

结论:

与Advax-CpG辅助的SARS-CoV-2 PsIV显示出免疫性和对SARS-CoV-2变种的潜在疗效.
作为一个独立的疫苗或作为NA疫苗的助推剂,SARS-CoV-2 PsIV显示出希望.
这种失活的全病毒疫苗策略可能有助于克服新出现的SARS-CoV-2变种引起的免疫逃避.

关键词:

阿德瓦克斯-CpGG 在线在 COVID-19 疫情中,这种疫苗是DNA疫苗.这就是SARS-CoV-2病毒.在SARS-CoV-2 PsIV.在SARS-CoV-2中和抗体中.符合性表征的表征.

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