对基于环共振器的生物传感器的表面功能化优化
Niccolò Ardoino1, Lorenzo Lunelli2,3, Georg Pucker2
1FTH S.r.l., Via Sommarive 18, I-38123 Trento, Italy.
Sensors (Basel, Switzerland)
|May 25, 2024
概括
微环共振器生物传感器的表面功能化使用aptamer和mercaptosilanes增强生物标志物检测. 这种优化的协议显示出对点护理液体活检应用的前景.
科学领域:
- 生物传感技术的技术
- 表面化学 表面化学
- 生物标志物检测检测 生物标志物检测
背景情况:
- 液体活检具有广泛临床应用的前景,由无标签生物传感器等点护理设备驱动.
- 有效的表面功能化对于生物传感器性能至关重要,确保准确,敏感和特定的分子检测.
研究的目的:
- 为了优化基于微环共振器 (MRR) 的生物传感器的表面功能,用于蛋白质生物标记物检测.
- 为敏感和特定的生物标志物识别开发一个强大的和可转移的功能化协议.
主要方法:
- 使用阿普坦和默卡普托西兰的MRR生物传感器表面的功能化.
- 优化等离子治疗 (),化 (1%v/v 默卡普托西兰),阿巴特马度 (1μM) 和固定时间 (3小时).
- 使用光子生物传感器进行血栓检测的功能化协议的表征和验证.
主要成果:
- 亚贡等离子处理和1%v/v的默卡普托西兰提供了适合合体结合的均表面.
- 优化了阿帕特马尔固定,实现了高性能.
- 功能化协议证明了对血栓的成功目标识别,尽管在现实条件下注意到了局限性.
结论:
- 开发的基于aptamer的表面功能化协议对MRR生物传感器是有效的.
- 该协议显示了敏感蛋白质生物标志物检测在护理点设置中的潜力.
- 需要进一步精细化,以解决临床转化实际测量条件的局限性.
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