微环共振器生物传感器用于检测SARS-CoV-2的核体蛋白
Yusuke Uchida1, Taro Arakawa1, Akio Higo2
1Graduate School of Engineering, Yokohama National University, 79-5 Tokiwada, Hodogaya-ku, Yokohama 240-8501, Japan.
Sensors (Basel, Switzerland)
|May 25, 2024
概括
一种新的微 (Si MRR) 生物传感器提供了对SARS-CoV-2核蛋白的快速和敏感检测. 这种光学生物传感器显示出早期检测的潜力,速度快,周转时间为10分钟.
科学领域:
- 光电学是指光电子产品.
- 生物感应是一种生物感应.
- 纳米技术 纳米技术
背景情况:
- 准确和快速检测SARS-CoV-2对于控制流行病至关重要.
- 现有的抗原测试可能耗时,敏感度各不相同.
研究的目的:
- 提出和演示一种高灵敏度微 (Si MRR) 光学生物传感器,用于检测SARS-CoV-2核体蛋白.
- 为了评估传感器的灵敏度,检测极限和速度.
主要方法:
- 在在绝缘体基板上制造Si MRR,使用CMOS兼容的工艺.
- 用针对选择性蛋白质吸附的抗体对Si MRR波导表面进行修改.
- 测量共振波长的变化,以量化蛋白质结合.
主要成果:
- MRR生物传感器获得了大约20,000的质量系数.
- 已证明可以选择性检测牛血清白蛋白和人类血清白蛋白.
- 初步结果表明,核囊的潜在灵敏度为10 pg/mL,检测时间小于10分钟.
结论:
- 开发的Si MRR光学生物传感器显示了对敏感和快速的SARS-CoV-2核蛋白检测的承诺.
- 传感器的性能与当前的抗原测试相当,但检测时间显著缩短.
- 预计进一步优化将提高其诊断能力.
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