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尼姆叶提取物表现出抗衰老和抗氧化作用,从酵母到人体细胞

Jinye Dang¹, Gongrui Zhang¹, Jingjing Li¹

¹Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.

|May 25, 2024

概括

尼姆叶提取物 (NLE) 延长寿命,增强酵母和人体细胞的氧化应激抵抗力. 它的抗衰老作用与氧化还原酶活性的调节有关,特别是通过CTT1基因.

科学领域:

生物遗传学生物遗传学
植物化学植物化学
分子生物学分子生物学

背景情况:

尼姆叶传统上用于长寿,但它们的抗衰老机制尚不清楚.
氧化应激是衰老的一个关键因素.
植物化学物质越来越多地研究它们的健康益处.

研究的目的:

为了研究尼姆树叶提取物 (NLE) 的抗衰老作用.
阐明NLE对寿命和氧化应激的影响背后的分子机制.
为了确定NLE中负责这些效应的活性化合物.

主要方法:

在Saccharomyces cerevisiae中的时间寿命测定.
氧化应激抵抗和反应性氧物种的测量.
用于化合物识别的LC/MS和GNPS.
复合目标网络建设 (STP,STITCH).
基因本体学 (GO) 和KEGG丰富分析.
在酵母中的RNA-seq分析.
对于反应性氧物种和衰老的HeLa细胞测定.

主要成果:

NLE延长了酵母的时间寿命,并改善了氧化应激抵抗力.
黄类化合物被确定为NLE的主要活性化合物.
NLE治疗上调调节了参与氧化还原酶活性的基因,特别是CTT1 (catalase).
在一个ctt1无突变酵母中,NLE的抗衰老和抗氧化作用被废除.
在人类HeLa细胞中,NLE减少了活性氧物种和衰老标志物.

结论:

尼姆树叶提取物具有显著的抗衰老和抗氧化特性.
部分通过调节氧化应激反应,通过涉及酶的途径来调节NLE的抗衰老作用.
NLE显示出作为抗衰老药物干预的植物化学品来源的潜力.

关键词:

阿扎迪拉赫塔表示这种植物是Saccharomyces cerevisiae.衰老的衰老衰老的衰老抗氧化剂是一种抗氧化剂.催化酶的催化剂是什么按时间顺序排列的寿命.网络药理学网络药理学

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