肠上皮质屏障功能受高血糖和二次胆汁酸在体外影响:特定的素的可能救援作用
Xin Tang1, Xiaochen Chen1, Michela Ferrari2
1Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, 9713 GZ, Groningen, the Netherlands.
Molecular nutrition & food research
|May 25, 2024
概括
特定的素,如DM19HB和DM43HB,可以帮助修复由糖尿病和炎症性肠病 (IBD) 等疾病引起的肠上皮质屏障破坏,特别是在高葡萄糖水平下.
科学领域:
- 胃肠病学 胃肠病学
- 细胞生物学 细胞生物学
- 微生物学 微生物学
背景情况:
- 肠表皮屏障的破坏是西方疾病的标志,如糖尿病和炎症性肠病 (IBD).
- 这种干扰导致肠道透性增加,炎症和肠道微生物群失调.
- 改变肠道微生物群的新陈代谢,特别是二次胆酸 (BAs),可能会影响肠道屏障功能和免疫力.
研究的目的:
- 调查食纤维的潜力,特别是点,以抵消肠道上皮质屏障的破坏.
- 为了比较pectin与二次胆汁酸 (BAs) 的不同甲基化和阻塞的作用.
- 在正常和高血糖条件下评估这些影响.
主要方法:
- 在T84肠上皮细胞中评估过体电阻 (TEER).
- 测量了紧密结节的基因表达.
- 在不同的葡萄糖度下 (5毫米和20毫米) 使用离子体A23187诱导屏障破坏.
- 暴露于二次胆酸 (LCA,DCA) 和不同类型的pectin (DM19HB,DM43HB) 的细胞.
主要成果:
- 非硫酸二次胆酸 (LCA和DCA) 对屏障完整性表现出显著的救援作用,特别是在20毫米葡萄糖下.
- 在两种葡萄糖条件下,特定的点类型DM19HB和DM43HB也表现出强大的屏障救援效应.
- 与5毫米葡萄糖相比,这些点在20毫米葡萄糖下表现出更强的疗效,这表明它们在控制高血糖引起的屏障功能障碍方面发挥了作用.
结论:
- 某些点结构 (DM19HB,DM43HB) 可以有效地恢复肠上皮质屏障功能.
- 这些素可以作为一种饮食替代品,以减轻与酸代谢障碍相关的障碍破坏.
- 研究结果强调了特定的食纤维在代谢疾病中管理肠道屏障完整性的治疗潜力.
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