使用全基因组测序来描述复发性结核病的病因学:阿拉斯加,2008-2020年
Yuri P Springer1, Megan L Tompkins2, Katherine Newell2,3
1Division of Tuberculosis Elimination, National Center for HIV, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia.
The Journal of infectious diseases
|May 25, 2024
概括
复发性结核病 (rTB) 在阿拉斯加的美国印第安人和阿拉斯加土著 (AIAN) 人群中经常再次感染. 全基因组测序 (WGS) 有效地识别了再感染,突出了加强监测和控制的必要性.
科学领域:
- 基因组学就是基因组学.
- 流行病学 流行病学
- 公共卫生 公共卫生
背景情况:
- 了解复发性结核病 (rTB) 的原因对于有效的控制策略至关重要.
- 传统的基因型定型方法在区分rTB病例中复发与再感染方面具有有限的分辨率.
- 全基因组测序 (WGS) 为rTB病因学研究提供了改进的遗传分辨率.
研究的目的:
- 从2008年到2020年,在阿拉斯加的美国印第安人和阿拉斯加土著 (AIAN) 人群中描述rTB的病因.
- 评估整个基因组单核酸多态性 (wgSNP) 距离和家族遗传学分析在确定rTB原因方面的实用性.
- 为了比较基于WGS的归因与传统的基因型鉴定方法.
主要方法:
- 应用 wgSNP 距离的系统评估和植物遗传学分析,对阿拉斯加 AIAN 个体的 rTB 病例进行了应用.
- 进行了监测数据的描述性分析和基于WGS的rTB病因学研究的文献搜索.
- 使用wgSNP距离,遗传学分析和常规基因型定型来比较重复发作与再感染的归因.
主要成果:
- 在阿拉斯加的AIAN人群中,结核病例的rTB率为11.8%,是全国平均水平的三倍.
- 通过遗传学分析的WGS将38例rTB病例中的65.8%归因于再感染,这是公布估计中最高的比例.
- 使用单独的wgSNP距离,再感染归因率为28.9%,使用常规基因类型测定,归因率为15.8%.
结论:
- 结合遗传距离和相关性标准,WGS有效阐明了rTB病因.
- 在阿拉斯加的AIAN人群中,高比例的rTB归因于再感染.
- 调查结果强调了在阿拉斯加的AIAN社区采取积极的监测和控制措施的重要性,以限制结核病的传播.
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