在行为/清醒的子的外层白色球体中进行细胞外记录的协议
Shiran Katabi1, Zvi Israel2, Hagai Bergman3
1Department of Medical Neuroscience, Institute of Medical Research Israel-Canada (IMRIC), The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
STAR protocols
|May 25, 2024
概括
这项研究详细介绍了一项在行为任务期间在子的外层白色球体 (GPe) 中进行细胞外记录的协议. 它可以检查与特定行为相关的神经元活动.
科学领域:
- 神经科学是一个神经科学.
- 电子生理学 电子生理学
- 灵长类动物研究研究
背景情况:
- 动物的细胞外记录将神经元活动与行为联系起来.
- 外部白色球体 (GPe) 在运动控制和学习中发挥作用.
- 了解GPe的功能需要方法来记录其在行为受试者中的活动.
研究的目的:
- 描述一项详细的协议,用于在行为子的GPe中执行细胞外记录.
- 为研究GPe神经元在任务执行过程中的电生理特性提供一种方法.
主要方法:
- 在子中进行室内和头部支架植入的立体性手术.
- 手术后的MRI扫描以确认GPe坐标和腔室位置.
- 在行为任务期间进行电生理学记录的数据收集程序.
主要成果:
- 描述的协议允许成功植入和记录在GPe.
- 可以从行为子的GPe神经元中获得电生理学数据.
- 该方法验证了针对GPe的准确性.
结论:
- 该协议为研究行为子GPe神经元活动提供了一种可靠的方法.
- 它有助于研究GPe在运动行为和相关认知功能中的作用.
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