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Updated: Jun 25, 2025

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
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CRISPR-Cas9编辑人类胚胎干细胞中的突触基因,用于诱导人类神经元中的功能分析

Aiden Houcek1, Z Zack Ma2, Brent Trauterman2

  • 1Department of Pharmacology, Vanderbilt University, Nashville, TN, USA; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA; Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA.

STAR protocols
|May 25, 2024
PubMed
概括

这项研究详细介绍了CRISPR-Cas9协议,用于制造具有特定基因突变的淘汰人类胚胎干细胞 (hESC),用于研究蛋白质功能和产生诱导神经元.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?神经科学 神经科学干细胞是干细胞的组成部分.

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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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