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在SCF/C-kit驱动的精子生成障碍是由皮辐射的abscopal效应诱导的小鼠脑膜辐射

Ling Guo¹, Tongzhou Qin¹, Xing Wang¹

¹Department of Radiation Protection Medicine, School of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, China; Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Xi'an 710032, China.

Ecotoxicology and environmental safety

|May 25, 2024

概括

部放射治疗通过氧化应激,炎症和荷尔蒙干扰导致男性不孕. 丸中的SCF/C-Kit通路激活驱动这个过程,影响精子生产.

科学领域:

生殖生物学生殖生物学
辐射瘤学辐射瘤学
分子毒理学分子毒理学

背景情况:

部放射治疗对于治疗白血病和脑瘤至关重要.
之前的研究表明,部辐射可以诱导导致精子生成障碍的腹膜效应.
这种疾病背后的精确分子机制尚不清楚.

研究的目的:

阐明骨辐射损害精子生成的分子机制.
为了确定关键的分子和信号通路参与辐射诱导的丸功能障碍.
调查氧化应激,炎症和下丘脑-垂体-阴茎轴在这个过程中的作用.

主要方法:

成年雄性C57BL/6小鼠接受了20 Gy的头骨X射线照射.
使用Tandem Mass Tag (TMT) 的丸蛋白质组分析在辐射后的1,2,4周进行.
生物信息学和基因本体学 (GO) 分析被用来识别受影响的途径和分子.

主要成果:

部辐射诱导严重的氧化应激和丸,血清和大脑的炎症.
观察到由下丘脑-垂体-淋巴结核 (HPG) 轴调节的性激素的干扰.
鉴定了丸PI3K/Akt通路的激活,由SCF/C-Kit升高驱动,以及精子干细胞功能的改变.

结论:

部辐射通过脑血丸级联导致精子生成障碍,其中包括氧化应激和炎症.
破坏HPG轴的分泌功能有助于丸功能障碍.
SCF/C-Kit/PI3K/Akt信号通路在调解辐射诱导的精子生成损伤方面发挥着至关重要的作用.

关键词:

无遮掩的效果无遮掩的效果部辐射辐射的治疗方法在SCF/C-kit中使用.精子发生障碍精子发生障碍测试 (Testis) 是一个测试.

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